Bacterial metabolism of 2-methylquinoline and naturally occurring 2-alkyl-4(1H) quinolones: (I) Transcriptional regulation of 2-methylquinoline degradation, (II) Bacterial strains and enzymes for the inactivation of 2-alkyl-4(1H)quinolones

2-甲基喹啉和天然存在的2-烷基-4(1H)喹诺酮类药物的细菌代谢：(I) 2-甲基喹啉降解的转录调控，(II) 用于灭活2-烷基-4(1H)喹诺酮类药物的细菌菌株和酶

基本信息

批准号：
94596318
负责人：
Professorin Dr. Susanne Fetzner
金额：
--
依托单位：
Institut für Molekulare Mikrobiologie und Biotechnologie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2009
资助国家：
德国
起止时间：
2008-12-31 至 2012-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/94596318?language=en
关键词：
Bacterial metabolism methylquinoline naturally occurring

项目摘要

2-Methylquinoline degradation by Arthrobacter nitroguajacolicus Rü61a to anthranilate is encoded by two “upper pathway” operons located on the linear plasmid pAL1. Anthranilate can be degraded via a chromosomal catechol pathway, however, another operon of pAL1 was proposed to encode “lower pathway” enzymes for anthranilate conversion via coenzyme A intermediates. Two PaaX-type regulators are involved in transcriptional control of the catabolic operons of pAL1, which in view of the current notion that PaaX proteins are restricted to the phenylacetate catabolon is an unexpected finding. The first part of the project aims at characterizing the transcriptional regulation of catabolic operons of pAL1. The following topics will be addressed: (i) Activities of catabolic promoters and of promoters of the paaX-like genes in response to carbon compounds; (ii) role of PaaX-like proteins in regulation of catabolic operons, and possible auto-regulation/reciprocal regulation of paaX genes by their gene products; (iii) identification of PaaX-responsive operator sequences, and effector specificity of the PaaX proteins; (iv) identification of other promoter-binding proteins; (v) characterization of pAL1-encoded genes for anthranilate conversion. The second section of the project addresses the question how bacteria degrade naturally occurring alkylquinolones, such as 2-heptyl-4(1H)quinolone and 2-heptyl-3-hydroxy-4(1H)quinolone, which are important quorum sensing signalling molecules of Pseudomonas aeruginosa. The goal is to identify novel enzymes for the inactivation of these compounds.

2-甲基喹啉降解由硝基核酸杆菌rü61a降解为炭疽菌，由位于线性质粒PAL上的两个“上途径”操纵子编码。炭疽菌可以通过染色体儿茶酚途径降解，但是，提议另一个PAL1的操纵子编码“下途径”酶通过辅酶A中间体进行炭疽菌转化。两个PAAX型调节剂参与了PAL1分解代谢操纵子的转录控制，鉴于当前的概念是Paax蛋白仅限于苯基乙酸可capababolon是一个出乎意料的发现。该项目的第一部分旨在表征PAL1分解代谢操纵子的转录调节。将解决以下主题：（i）响应碳化合物的分解代谢启动子和Paax样基因的启动子的活性；（ii）PAAX样蛋白在分解代谢操作元调节中的作用，以及通过其基因产物对PAAX基因的自身调节/相互调节；（iii）鉴定PAAX响应性算子序列和PAAX蛋白的效应特异性；（iv）鉴定其他启动子结合蛋白；（v）对PAL1编码的基因进行炭疽菌转化的表征。该项目的第二部分解决了一个问题，细菌如何降解自然存在的烷基喹诺酮类（例如2- heptyl-4（1H）喹诺酮类和2-羟基-3-羟基-4（1H）奎诺酮，它们是重要的Quorum quorum seming Quorum感应信号分子，这些奎诺酮是pseudomononas erecunas ereginassosa的重要Quorum传感。目的是确定新的酶以使这些化合物失活。