Cell & Molecular Imaging Shared Resource

细胞与分子影像共享资源：综述 细胞与分子成像共享资源(CMI)的任务是为霍林斯癌症中心提供 拥有最先进的基于细胞的成像和分析技术、专业知识和培训的成员。 提供的成像平台包括实时和固定的共焦、多光子和超分辨率显微镜 细胞和组织、活体成像、自动成像和组织切片图像量化。CMI 配备蔡司LSM 880 NLO多光子/共焦/Airyscan超分辨率，奥林巴斯FluoView FV1200 活体多光子，蔡司LSM 510元共焦，奥林巴斯FluoView FV 10i Liv活细胞共焦，以及BD 生物科学公司CARV II旋转圆盘共焦显微镜和新型Perkin-Elmer Vectra Polaris自动化仪 定量病理成像系统。显微镜配备了环境室，用于 温度和CO2/O2控制，以实现活细胞的高分辨率无损3D成像 有机体。应用包括：1)用于监测离子的参数敏感荧光团的活细胞成像， 电位，自由基产生，吡啶核苷酸还原，膜通透性，细胞存活率 (细胞凋亡和坏死)、荧光蛋白标记等荧光记者；2)高分辨率 免疫细胞化学和荧光蛋白分布的组织切片成像；3)荧光 共振能量转移(FRET)和双链接来表征和量化分子间相互作用；4) 活体显微镜监测微循环、白细胞边集、线粒体极化和 活体动物的渗透性和其他因素；以及5)高通量、定量多路复用成像 常规和免疫染色标本。辅助设备包括组织培养罩、孵化器 以及用于多孔培养细胞平行测量的荧光和吸光度平板读取器 车牌。通过病理和检验医学系，CMI还提供咨询， 组织病理学(冰冻切片、石蜡切片和免疫细胞化学)方面的培训和服务 相关电子显微镜。CMI服务包括理论和实践培训以及帮助 初级调查员。运行Imaris、ImageJ斐济、Metamorph和其他软件的成像工作站提供 离线图像处理和分析。在当前周期中，CMI支持了54个肝细胞癌实验室的研究，导致 代表所有肝细胞癌研究项目的23名研究人员发表了40篇论文，提交了50份拨款 提案，包括P20生物医学研究卓越中心(Cobre)关于“氧化剂，氧化还原平衡， 和压力信号“(P20 GM103542)，以及肝脏和肝脏疾病COBRE的未决申请 胃肠道和P30消化系统疾病研究核心中心。在约翰·J.的领导下。 莱马斯特斯，医学博士，线粒体和能量学生物学的国家领导者，CMI共享资源 发展成为可视化和量化细胞过程的极好的科学资源。

CELL & MOLECULAR IMAGING SHARED RESOURCE: SUMMARY The mission of the Cell & Molecular Imaging Shared Resource (CMI) is to provide Hollings Cancer Center (HCC) members with technologies, expertise, and training for state-of-the-art cell-based imaging and analysis. Imaging platforms provided include confocal, multiphoton, and super-resolution microscopy of live and fixed cells and tissues, intravital imaging, automated imaging, and image quantification of histological slides. CMI houses Zeiss LSM 880 NLO multiphoton/confocal/Airyscan super-resolution, Olympus Fluoview FV1200 intravital multiphoton, Zeiss LSM 510 META confocal, Olympus Fluoview FV 10i LIV live cell confocal, and BD BioSciences CARV II spinning disk confocal microscopes, and a new Perkin-Elmer Vectra Polaris Automated Quantitative Pathology Imaging System. Microscopes are equipped with environmental chambers for temperature and CO2/O2 control to allow high-resolution non-destructive 3D imaging of living cells and organisms. Applications include: 1) live cell imaging of parameter-sensitive fluorophores to monitor ions, electrical potentials, radical generation, pyridine nucleotide reduction, membrane permeability, cell viability (apoptosis and necrosis), fluorescent protein labeling, and other fluorescent reporters; 2) high resolution imaging of tissue sections for immunocytochemistry and fluorescent protein distribution; 3) fluorescence resonance energy transfer (FRET) and Duolink to characterize and quantify intermolecular interactions; 4) intravital microscopy to monitor microcirculation, leukocyte margination, mitochondrial polarization and permeability, and other factors in living animals; and 5) high throughput, quantitative multiplexed imaging of conventionally and immunostained specimens. Ancillary equipment includes tissue culture hoods, incubators and fluorescence and absorbance plate readers for parallel measurements in cultured cells grown on multi-well plates. Through the Department of Pathology and Laboratory Medicine, CMI also provides consultation, training, and services in histopathology (frozen and paraffin sections and immunocytochemistry) and correlative electron microscopy. CMI services include theoretical and hands-on training and assistance to junior investigators. Imaging workstations running Imaris, ImageJ FIJI, Metamorph, and other software provide offline image processing and analysis. In the current cycle, CMI supported research in 54 HCC labs leading to 40+ publications by 23 investigators representing all HCC research programs and submission of 50+ grant proposals, including a P20 Center of Biomedical Research Excellence (COBRE) on “Oxidants, Redox Balance, and Stress Signaling” (P20 GM103542), plus pending applications for a COBRE in Diseases of the Liver and Gastrointestinal Tract, and a P30 Digestive Diseases Research Core Center. Under the leadership of John J. Lemasters, MD, PhD, a national leader in mitochondria and energetics biology, the CMI Shared Resource has grown to become a superb scientific resource for the visualization and quantification of cellular processes.