Cell & Molecular Imaging Shared Resource

细胞

• 批准号: 10377468
• 负责人: John J Lemasters
• 金额: $ 8.69万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10377468
• 关键词: 3-Dimensional; Address; Animals; Apoptosis; Applications Grants; Area; Basic Science; Biological Sciences; Biology; Cancer Center; Cancer Center Support Grant; Cancer Patient; Carbon Dioxide; Cell Membrane Permeability; Cell Survival; Cell physiology; Cells; Centers of Research Excellence; Clinical; Clinical Research; Clinical Trials; Combined Modality Therapy; Complex; Computer software; Conceptions; Confocal Microscopy; Consult; Consultations; Cultured Cells; Data Set; Detection; Development; Digestive System Disorders; Doctor of Philosophy; Educational workshop; Electron Microscopy; Emerging Technologies; Ensure; Equilibrium; Equipment; Event; Fluorescence; Fluorescence Resonance Energy Transfer; Freezing; Future; Gastrointestinal Diseases; Generations; Goals; Heterogeneous-Nuclear Ribonucleoproteins; Histopathology; Image; Image Analysis; Imaging technology; Immunotherapy; Incubators; Interleukin-15; Ions; Label; Laboratories; Lasers; Leadership; Leukocytes; Liver diseases; Malignant Neoplasms; Measurement; Medical; Medicine; Microcirculation; Microscope; Microscopy; Mission; Mitochondria; Monitor; NCI Center for Cancer Research; Necrosis; Non-Small-Cell Lung Carcinoma; Organism; Oxidants; Oxidation-Reduction; Paraffin; Pathology; Patient Care; Patients; Permeability; Proteins; Protocols documentation; Publications; Reader; Regimen; Reporter; Research; Research Personnel; Research Project Grants; Research Support; Resolution; Resource Sharing; Resources; Role; Running; Series; Services; Signal Transduction; Slide; South Carolina; Specimen; Strategic Planning; Stress; Structure; System; Technology; Temperature; Textiles; Therapeutic; Three-Dimensional Image; Three-Dimensional Imaging; Three-dimensional analysis; Tissue imaging; Tissues; Training; Training Activity; Training and Education; Translational Research; Tumor Biology; Universities; Visualization; Visualization software; Warburg Effect; anti-PD-1; base; beta-arrestin; cellular imaging; deep learning; digital imaging; electrical potential; fluorophore; high resolution imaging; histological slides; histological specimens; image processing; imaging Segmentation; imaging capabilities; imaging platform; imaging system; immunocytochemistry; immunotherapy clinical trials; improved; instrument; instrumentation; intermolecular interaction; intravital imaging; intravital microscopy; learning strategy; lectures; light microscopy; liquid crystal polymer; live cell imaging; malignant breast neoplasm; meetings; member; microscopic imaging; mitochondrial metabolism; molecular imaging; multi-photon; multiphoton microscopy; multiplexed imaging; novel; pathology imaging; preclinical study; programs; protein distribution; pyridine nucleotide; quasar; ranpirnase; temporal measurement; therapeutic effectiveness; tissue culture; tool; tumor

CELL & MOLECULAR IMAGING SHARED RESOURCE: SUMMARY The mission of the Cell & Molecular Imaging Shared Resource (CMI) is to provide Hollings Cancer Center (HCC) members with technologies, expertise, and training for state-of-the-art cell-based imaging and analysis. Imaging platforms provided include confocal, multiphoton, and super-resolution microscopy of live and fixed cells and tissues, intravital imaging, automated imaging, and image quantification of histological slides. CMI houses Zeiss LSM 880 NLO multiphoton/confocal/Airyscan super-resolution, Olympus Fluoview FV1200 intravital multiphoton, Zeiss LSM 510 META confocal, Olympus Fluoview FV 10i LIV live cell confocal, and BD BioSciences CARV II spinning disk confocal microscopes, and a new Perkin-Elmer Vectra Polaris Automated Quantitative Pathology Imaging System. Microscopes are equipped with environmental chambers for temperature and CO2/O2 control to allow high-resolution non-destructive 3D imaging of living cells and organisms. Applications include: 1) live cell imaging of parameter-sensitive fluorophores to monitor ions, electrical potentials, radical generation, pyridine nucleotide reduction, membrane permeability, cell viability (apoptosis and necrosis), fluorescent protein labeling, and other fluorescent reporters; 2) high resolution imaging of tissue sections for immunocytochemistry and fluorescent protein distribution; 3) fluorescence resonance energy transfer (FRET) and Duolink to characterize and quantify intermolecular interactions; 4) intravital microscopy to monitor microcirculation, leukocyte margination, mitochondrial polarization and permeability, and other factors in living animals; and 5) high throughput, quantitative multiplexed imaging of conventionally and immunostained specimens. Ancillary equipment includes tissue culture hoods, incubators and fluorescence and absorbance plate readers for parallel measurements in cultured cells grown on multi-well plates. Through the Department of Pathology and Laboratory Medicine, CMI also provides consultation, training, and services in histopathology (frozen and paraffin sections and immunocytochemistry) and correlative electron microscopy. CMI services include theoretical and hands-on training and assistance to junior investigators. Imaging workstations running Imaris, ImageJ FIJI, Metamorph, and other software provide offline image processing and analysis. In the current cycle, CMI supported research in 54 HCC labs leading to 40+ publications by 23 investigators representing all HCC research programs and submission of 50+ grant proposals, including a P20 Center of Biomedical Research Excellence (COBRE) on “Oxidants, Redox Balance, and Stress Signaling” (P20 GM103542), plus pending applications for a COBRE in Diseases of the Liver and Gastrointestinal Tract, and a P30 Digestive Diseases Research Core Center. Under the leadership of John J. Lemasters, MD, PhD, a national leader in mitochondria and energetics biology, the CMI Shared Resource has grown to become a superb scientific resource for the visualization and quantification of cellular processes.

细胞和分子成像共享资源：摘要 细胞和分子成像共享资源（CMI）的使命是为Hollings癌症中心提供 (HCC)成员拥有技术、专业知识和最先进的基于细胞的成像和分析培训。 提供的成像平台包括共焦，多光子和超分辨率显微镜的活的和固定的 细胞和组织、活体成像、自动成像和组织学载玻片的图像定量。CMI 拥有Zeiss LSM 880 NLO多光子/共焦/Airyscan超分辨率，Olympus Fluoview FV 1200 活体多光子、Zeiss LSM 510 Meta共聚焦、Olympus Fluoview FV 10 i LIV活细胞共聚焦和BD BioSciences CARV II旋转圆盘共聚焦显微镜和全新的Perkin-Elmer Vectra Polaris Automated 定量病理成像系统。显微镜配备了环境室， 温度和CO2/O2控制，以实现活细胞的高分辨率非破坏性3D成像， 有机体应用包括：1）参数敏感荧光团的活细胞成像以监测离子， 电位，自由基生成，吡啶核苷酸还原，膜通透性，细胞活力 （凋亡和坏死），荧光蛋白标记和其他荧光报告; 2）高分辨率 用于免疫细胞化学和荧光蛋白分布的组织切片成像; 3）荧光 共振能量转移（FRET）和Duolink来表征和量化分子间相互作用; 4） 活体显微镜检查以监测微循环、白细胞边集、线粒体极化和 渗透性和活动物中的其他因素;和5）高通量、定量多路成像， 常规和免疫染色标本。辅助设备包括组织培养罩、培养箱 以及荧光和吸光度板读数器，用于在多孔上生长的培养细胞中进行平行测量 板.通过病理学和实验室医学系，CMI还提供咨询， 组织病理学培训和服务（冰冻切片和石蜡切片以及免疫细胞化学）， 相关电子显微术CMI服务包括理论和实践培训， 初级调查员运行Imaris、ImageJ FIJI、Metamorph和其他软件的成像工作站提供 离线图像处理和分析。在当前周期中，CMI支持了54个HCC实验室的研究， 23名研究者代表所有HCC研究项目发表了40多篇论文，并提交了50多份研究基金 建议，包括P20生物医学研究卓越中心（COBRE）的“氧化剂，氧化还原平衡， 和应激信号传导”（P20 GM 103542），加上肝脏疾病中COBRE的未决申请， 胃肠道和P30消化系统疾病研究核心中心。在约翰·J。 Lemasters，医学博士，博士，线粒体和能量生物学的国家领导人，CMI共享资源有 发展成为可视化和量化细胞过程的极好的科学资源。