Biospecimen-Core

生物样本核心

• 批准号: 10385163
• 负责人: SAYEED IKRAMUDDIN
• 金额: $ 27.57万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10385163
• 关键词: Biospecimen; Core

Project Summary Senescent cells (SnCs) accumulate with age and in various chronic disease states and clearly contribute to driving morbidity and mortality in model organisms. Drugs that selectively eliminate SnCs (“senolytics”) were first developed by our team and have been demonstrated to reduce frailty, extend median lifespan, and attenuate morbidity in murine models of numerous age-related diseases. However, SnCs also play a role in wound healing and tissue remodeling. To advance senolytics to their greatest potential, then, it is imperative to understand more about SnCs in humans with healthy aging. As part of SenNet, the proposed Minnesota Tissue Mapping Center (MN TMC) has selected four tissues for identification, characterization, and spatio-temporal analysis of senescence: adipose, skeletal muscle, liver, and ovarian tissues. These tissues were selected because of the scientific expertise at UMN and Mayo Clinic in the biology of these organs, evidence of increased senescence with age, and availability of biobanked specimens as well as surgical access to fresh tissues from healthy individuals. The overall goal of the Biospecimen Core (BSP), co-directed by Dr. Sayeed Ikramuddin, Chair of the UMN Department of Surgery, and Dr. Oyedele Adeyi, Professor of Laboratory Medicine and Pathology, is to provide the tissues and blood for PBMC isolation to the Biological Analysis Core (BAC) for bulk, single cell, and spatial analysis of human SnCs for subsequent multi-plex analysis of the results by the Data Analysis Core (DAC). The BSP will leverage their strong track record of obtaining high-quality, well-annotated human samples from non-chronically diseased individuals who are metabolically characterized and with appropriate diversity in gender, race, socio-economic status, ethnicity, and other relevant diversity parameters. The BSP will also provide access to UMN’s specimen procurement network (BioNet) that has ~70,000 archival tissues (frozen, FFPE) and biofluids (frozen) collected for research, including several adipose depots, several muscles, liver from organ donors, and healthy ovarian tissues removed because of benign lesions. In addition, the BSP has access to tissues within the Rochester Epidemiology Project, which encompasses the medical records of all citizens living in Olmsted County, MN since 1966, along with archived biological specimens. The Aims of the BSP are to: 1) Prepare and maintain all regulatory materials supporting human tissue collection; 2) Recruit and consent patients of different ages for this project; 3) Characterize the metabolic health of enrolled participants; 4) Coordinate with BioNet for intraoperative specimen collection, annotation, and storage of tissues; 5) Procure and annotate high quality target tissues along with relevant biofluids; 6) Longitudinally follow patients for which weight loss and bariatric surgery are metabolic perturbations; 7) Disseminate the samples to MN TMC BAC; and 8) Partner with SenNet to share tissues and to develop shared patient consent and tissue collection protocols with appropriate Ethical, Legal and Social Implications and quality control and assurance considerations.

项目摘要 衰老细胞（SnC）随着年龄的增长和各种慢性疾病状态而积累，并明显有助于 导致模式生物的发病率和死亡率选择性消除SnCs的药物（“senolytics”）首先被用于治疗癌症。 由我们的团队开发，并已被证明可以减少虚弱，延长中位寿命， 许多年龄相关疾病的鼠模型中的发病率。然而，SnCs也在伤口愈合中发挥作用 和组织重塑。为了推进senolytics发挥其最大的潜力，那么，有必要了解更多 关于SnCs在人类健康衰老中的作用。作为SenNet的一部分，拟议的明尼苏达组织绘图中心 (MN TMC）选择了四种组织进行识别，表征和时空分析， 衰老：脂肪、骨骼肌、肝脏和卵巢组织。选择这些组织是因为 UMN和马约诊所在这些器官的生物学方面的科学专业知识， 随着年龄的增长，生物库标本的可用性以及手术获取健康人的新鲜组织， 个体生物样本核心（BSP）的总体目标，由主席Sayeed Ikramuddin博士共同指导， UMN外科系和实验室医学和病理学教授Oyedele Adeyi博士是 向生物分析中心（BAC）提供用于PBMC分离的组织和血液，用于批量、单细胞， 人类SnCs的空间分析，用于数据分析核心对结果的后续多重分析 （发援会）。BSP将利用其强大的轨道 获得高质量、注释良好的人体样本的记录 来自非慢性疾病个体，这些个体具有代谢特征并且在以下方面具有适当的多样性： 性别、种族、社会经济地位、种族和其他相关多样性参数。BSP还将 提供访问 UMN的标本采购网络（BioNet）拥有约70，000个存档组织（冷冻， FFPE）和收集用于研究的生物流体（冷冻），包括几个脂肪库、几块肌肉、来自 器官捐赠者和因良性病变而切除的健康卵巢组织。此外，BSP还可以访问 罗切斯特流行病学项目中的组织，该项目包括所有公民的医疗记录 自1966年起居住在明尼苏达州奥姆斯特德县，沿着的还有存档的生物标本。BSP的目标是 目的：1）准备和维护支持人体组织采集的所有监管材料; 2）招募和同意 本项目不同年龄段的患者; 3）描述入选参与者的代谢健康状况; 4） 与BioNet协调术中标本采集、注释和组织保存; 5）采集和 用相关的生物流体注释高质量的靶组织沿着; 6）纵向跟踪患者， 损失和减肥手术是代谢紊乱; 7）将样品传播到MN TMC BAC;和8） 与SenNet合作，共享组织，并制定共享的患者同意书和组织收集协议， 适当的道德、法律的和社会影响以及质量控制和保证考虑。