Updated confocal microscope in the Imaging Core facility to support biomedical research

P20GM103653核心设施设备补充 摘要 特拉华州立大学神经科学中心附属教员也 OSCAR成像核心工具的主要用户，该工具由 特拉华州因布雷。然而，目前运行的两台共焦显微镜 在设施中可以追溯到上个世纪，以及图像以缓慢的速度 收集的数据限制了它们在活细胞和定量成像中的应用 并限制教员可以使用这些显微镜解决的研究问题。收购一台旋转磁盘 系统共聚焦显微镜将大大加强和加强研究和教育活动 特拉华州立大学(DSU)，一所以本科和历史黑人为主的大学。获得专利的 旋转圆盘系统将带来共焦和延时显微镜的显著改进，具有更好的空间分辨率 高分辨率生物成像的分辨率、时间分辨率和改进的信噪比。显微镜 将安装在OSCAR成像设施中，这是一个多用户共享设施，允许不同的用户池访问 这项先进的技术。 我们将用NIGMS购买Voxyl旋转盘系统(V3共焦头和附件) 补充资金，以及一台IX83奥林巴斯倒置显微镜(显微镜支架和附件)，带 这些资金将被用来建造一个高分辨率的共焦显微镜。这个 大会将由Boukari博士指导，他是一位在光学和光学领域拥有丰富经验的光学物理学家 成像仪器，担任奥斯卡成像核心主任。布卡里博士已经 他之前在NIH工作时组装了一个类似的显微镜。他将得到我们核心部门的协助 设施经理，他有10年使用和维护共焦显微镜的经验。提供资金 这一补充将使DSU能够利用我们的机构资金以及我们成像的专业知识和诀窍 工厂员工将引导我们的方式，使尖端技术共焦显微镜可用于我们的 神经科学和生物医学研究人员。通过自己组装系统，我们将节省超过150,000美元 与购买交钥匙系统相比。

Core Facility Equipment Supplement to P20GM103653 Summary Neuroscience-Center affiliated faculty at Delaware State University are also major users of the OSCAR Imaging Core Facility that is supported by the Delaware INBRE. However, the two confocal microscopes currently operating in the facility date from the previous century, and the slow rate at which image data are collected limits their application for live-cell and quantitative imaging and limit the research questions that faculty can address using those microscopes. The acquisition of a spinning-disk system confocal microscope will enhance and strengthen significantly the research and educational activities at Delaware State University (DSU), a primarily undergraduate and Historically-Black university. The patented spinning-disk system will bring a significant improvement in confocal and time-lapse microscopy with better spatial- resolution, temporal-resolution, and improved signal-to-noise ratios for high-resolution bioimaging. The microscope will be installed in the OSCAR Imaging Facility, a multi-user shared facility, allowing diverse pool of users to access this advanced technology. We will acquire a Voxyl Spinning-Disk system (V3 confocal head and accessories) with NIGMS supplement funds, and an IX83 Olympus inverted microscope (microscope stand and accessories) with DSU institutional funds, which will be assembled to build a high-resolution confocal microscope. The assembly will be directed by Dr. Boukari, an optical physicist with extensive experience in optical and imaging instrumentation who serves as the director of the OSCAR Imaging Core. Dr. Boukari has previously assembled a similar microscope while he was working at NIH. He will be assisted by our Core Facility Manager, who has 10 years of experience using and maintaining confocal microscopes. Funding of this supplement will allow DSU to leverage our institutional funds and the expertise and know-how of our Imaging Facility staff to “bootstrap” our way to making cutting edge technology confocal microscopy available to our neuroscience and biomedical researchers. By assembling the system ourselves we will save more than $150,000 compared to purchasing a turn-key system.