ALS Efficiently Networking Advanced Beam Line Experiments (ALS-ENABLE)

总体摘要/摘要 这项建议的总体目标是继续提供综合的、高效的同步加速器结构生物学 为研究界提供资源。该资源名为ALS-Enable，位于Advanced Light 来源(ALS)，加利福尼亚州伯克利。该团队有二十年操作大分子X射线的经验 结晶学(MX)和小角X射线散射(SAXS)光束线，以及最近的X射线 足迹质谱仪(XFMS)光束线。在过去的四年里，这个团队一直密切合作 为了创建ALS使能资源，许多团队成员都在三次X光方面进行了交叉培训 结构生物学方法。在过去的4年中，我们实现了对ALS结构的透明接口 生物资源，并帮助用户在常规和具有挑战性的情况下进行成功的结构确定 有问题。我们与各种各样的用户社区合作，从专家到同步加速器的新用户，都有 结构生物学技术方面的培训有限。在必要的地方，我们已经引导用户通过最多的 回答他们的生物学问题的适当途径。在这份续签申请中，我们建议提出几项 随着结构生物学领域最近的变化，资源的变化，利用新的高度- 性能光束线(双子座)，并结合了现已成熟的X射线足迹同步加速器技术 (XFMS)。

Overall Summary/Abstract The overall goal of this proposal is to continue to provide an integrated, efficient synchrotron structural biology Resource to the research community. This Resource, called ALS-ENABLE, is located at the Advanced Light Source (ALS) in Berkeley, California. The team has two decades of experience operating macromolecular X-ray crystallography (MX) and small angle X-ray scattering (SAXS) beamlines, and more recently, an X-ray footprinting mass spectrometry (XFMS) beamline. The team has worked closely together over the last 4 years to create the ALS-ENABLE Resource, and many of the team members are cross-trained in the three X-ray structural biology methods. During the 4 years we have implemented a transparent interface to the ALS structural biology resources, and helped users pursue successful structure determination for both routine and challenging problems. We have worked with a diverse user community, ranging from experts to new synchrotron users with limited training in structural biology techniques. Where necessary we have guided users through the most appropriate routes for answering their biological question. In this renewal application we propose to make several changes to the Resource in response to recent changes in the field of structural biology, leverage a new high- performance beamline (GEMINI), and incorporate the now mature synchrotron technique of X-ray footprinting (XFMS).