Pilatus 6m

 描述(申请人提供)：伯克利结构生物学中心(BCSB)在高级光源上运营着五条大分子晶体光束线，目前正在建造一条新的微焦点光束线Gemini。新的光束线将提供比目前的光束线高一个数量级的通量，用户可以聚焦到10微米以下。双子座正在建设中，以满足该国目前对高亮度小光束结晶学的需求；目前的微焦光束线供过于求，远远超过能力。通过双子座，我们的目标是通过为全国各地由NIH资助的研究人员提供最先进的结构生物学工具来缓解这种需求。虽然双子座将使用最先进的自动化、软件处理和硬件控制，但它还没有资金用于高端探测器系统，以匹配光束线的其他能力。基于像素阵列的直接光子计数探测器在过去几年中取得了巨大的进步，并在国家用户设施中变得越来越常见。这些系统在速度、量子效率、灵敏度和动态范围方面的增益比大多数当前的CCD探测器都是显著的，事实上对于许多结构生物学项目的成功至关重要。高速实现了数据采集的精细PHI切片模式，同时将辐射损害降至最低，并允许极高的吞吐量，从而可以分析更多的样品。大的动态范围意味着可以同时收集强反射和弱反射，这也将辐射损害降至最低，并增加了吞吐量。由于非常小的点扩散函数，可以在单个像素上捕获反射的事实有效地减小了探测器上的光斑大小。我们的目标是在双子座上安装一个高端像素阵列探测器，双子座将有超过75%的时间为NIH支持的研究人员服务。

 DESCRIPTION (provided by applicant): The Berkeley Center for Structural Biology (BCSB) operates five macromolecular crystallography beamlines at the Advanced Light Source, and is now building a new microfocus beamline, Gemini. The new beamline will provide an order of magnitude higher flux than the current beamlines, in a user- focusable beam down to 10 microns. Gemini is being built to address the current need for high- brightness small-beam crystallography in the country; the current microfocus beamlines are oversubscribed, and demand far outstrips capacity. With Gemini, we aim to alleviate this need by supplying a state-of-the-art structural biology tool for NIH-funded investigators around the country. While Gemini will use the most state-of-the art automation, software processing, and hardware control, it as yet does not have funding for a high-end detector system to match the other capabilities of the beamline. Detectors based on pixel-arrays for direct photon counting have made huge advances in the last few years, and have become increasingly common at national user facilities. The gain in speed, quantum efficiency, sensitivity, and dynamic range of these systems over most current CCD detectors is significant, and in fact critical to the success of many structural biology projects. The high speed enables fine phi slicing mode of data collection while minimizing radiation damage, and allows extremely high throughput so that many more samples can be analyzed. The large dynamic ranges means that both strong and weak reflections can be collected simultaneously, which also minimizes radiation damage and increases throughput. The fact that a reflection can be captured on a single pixel, due to the very small point spread function, effectively reduces spot size on the detector. Our goal is to install a high-end pixel-array detector on Gemini, which will have over 75% of its time available to serve NIH-supported researchers.