ALS Efficiently Networking Advanced Beam Line Experiments (ALS-ENABLE)

总体总结/摘要 该提案的总体目标是继续提供集成、高效的同步加速器结构生物学 为研究界提供资源。该资源称为 ALS-ENABLE，位于 Advanced Light 来源（ALS）位于加利福尼亚州伯克利。团队拥有二十年高分子X射线操作经验 晶体学 (MX) 和小角 X 射线散射 (SAXS) 光束线，以及最近的 X 射线 足迹质谱 (XFMS) 光束线。该团队在过去 4 年里密切合作 创建 ALS-ENABLE 资源，许多团队成员都接受了三种 X 射线的交叉培训 结构生物学方法。在 4 年里，我们实现了 ALS 结构的透明接口 生物学资源，并帮助用户成功确定常规和具有挑战性的结构 问题。我们与多元化的用户社区合作，从专家到新的同步加速器用户 结构生物学技术方面的培训有限。如有必要，我们会引导用户完成最 回答他们的生物学问题的适当途径。在此续展申请中，我们建议提出几项 为了响应结构生物学领域的最新变化而对资源进行了更改，利用了新的高 性能光束线（GEMINI），并结合了现已成熟的 X 射线足迹同步加速器技术 （XFMS）。

Overall Summary/Abstract The overall goal of this proposal is to continue to provide an integrated, efficient synchrotron structural biology Resource to the research community. This Resource, called ALS-ENABLE, is located at the Advanced Light Source (ALS) in Berkeley, California. The team has two decades of experience operating macromolecular X-ray crystallography (MX) and small angle X-ray scattering (SAXS) beamlines, and more recently, an X-ray footprinting mass spectrometry (XFMS) beamline. The team has worked closely together over the last 4 years to create the ALS-ENABLE Resource, and many of the team members are cross-trained in the three X-ray structural biology methods. During the 4 years we have implemented a transparent interface to the ALS structural biology resources, and helped users pursue successful structure determination for both routine and challenging problems. We have worked with a diverse user community, ranging from experts to new synchrotron users with limited training in structural biology techniques. Where necessary we have guided users through the most appropriate routes for answering their biological question. In this renewal application we propose to make several changes to the Resource in response to recent changes in the field of structural biology, leverage a new high- performance beamline (GEMINI), and incorporate the now mature synchrotron technique of X-ray footprinting (XFMS).