Husbandry and Management

畜牧与管理

• 批准号: 10447030
• 负责人: Melween I. Martinez
• 金额: $ 256.44万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10447030
• 关键词: Accreditation; Acquired Immunodeficiency Syndrome; Alleles; Animal Testing; Animals; Biomedical Research; Breeding; Budgets; Caribbean region; Caring; Characteristics; Communities; Ensure; Female; Funding; Funding Mechanisms; Genetic; Goals; Grant; Health; Heterozygote; IACUC; Inbreeding; Income; Institution; Laboratory Animals; Macaca mulatta; Medical; Monitor; Monkeys; Population Genetics; Primates; Puerto Rico; Recovery; Regulation; Reproduction; Research; Research Personnel; Research Project Grants; Resources; SIV; Safety; Sales; Science; Services; Site Visit; Solid; Stratification; Testing; Time; United States Department of Agriculture; United States National Institutes of Health; Universities; Viral; Virus; Visit; Work; animal care; animal colony; base; cohort; cost; genetic analysis; germ free condition; male; offspring; operation; programs

PROJECT SUMMARY/ABSTRACT: The major goal of this application is to sustain the Specific Pathogen Free (SPF) program at the Caribbean Primate Research Center's (CPRC) through support of operations, administration, veterinary care, virological and MHC typing and by the improvement of our comprehensive genetic management plan. A unique feature of CPRC animals in favoring a successful establishment and sustainable of SPF colony are that these animals are naturally free of SIV and SRV-D, two of the four viruses to be tested for. Nevertheless, for safety and as required for the SPF Program, we continue systematically testing for all four viruses (B virus, STLV, SIV and SRV-D). The Program provides unadmixed Indian-origin rhesus monkeys with defined genetic and virological background. The population genetic analyses revealed that the SPF colony has remained genetically homogenous over time with sufficient amounts of heterozygosity and minimal stratification from its non-SPF founders. We plan to attain the following specific aims during the execution of the proposed plan in this Core: Aim 1: To maintain the closed CPRC- SPF indian-origin rhesus macaques colony, at a steady state level, that can provide high quality animals to support numerous NIH funded research projects, and to continue working toward self- sufficiency. The SPF colony will be maintained in compliance with the regulatory bodies. Aim 2. To use the projected recovery cost income, as part of an integral financial plan, to support the CPRC-SPF colony. With continued NIH support, the Institutional contribution and with funds from the program income, the SPF program at CPRC will remain a significant national research resource and will be able to provide healthy SPF indian-origin rhesus monkeys of defined genetic and virological background to NIH-sponsored research programs.

项目总结/摘要： 这项申请的主要目标是维持加勒比地区的无特定病原体（SPF）计划 灵长类动物研究中心（CPRC）通过支持运营，管理，兽医护理，病毒学 和MHC分型，并通过改进我们的综合遗传管理计划。的独特特征 CPRC动物有利于成功建立和可持续的SPF群体，这些动物是 天然不含SIV和SRV-D，这是四种待检测病毒中的两种。然而，为了安全和必要的 对于SPF计划，我们继续系统地检测所有四种病毒（B病毒、STLV、SIV和SRV-D）。的 计划提供具有明确遗传和病毒学背景的印度恒河猴。 群体遗传分析显示，SPF群体随着时间的推移保持遗传同质性 具有足够量的杂合性和来自其非SPF建立者的最小分层。 我们计划在执行本核心建议计划期间实现以下具体目标：目标1： 维持封闭的CPRC-SPF印度恒河猴群体处于稳定状态， 高质量的动物，以支持许多NIH资助的研究项目，并继续努力实现自我- 充足性SPF菌落将按照监管机构的规定进行维护。目标2.使用 预计回收成本收入，作为整体财务计划的一部分，以支持CPRC-SPF殖民地。 在NIH的持续支持下，机构捐款和来自计划收入的资金，SPF CPRC的一项计划将仍然是一个重要的国家研究资源，并将能够提供健康的SPF NIH赞助的研究中确定遗传和病毒学背景的印度恒河猴 程序.