Husbandry and Management

畜牧与管理

• 批准号: 10217278
• 负责人: Melween I. Martinez
• 金额: $ 252.45万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10217278
• 关键词: Accreditation; Acquired Immunodeficiency Syndrome; Alleles; Animal Testing; Animals; Biomedical Research; Breeding; Budgets; Caribbean region; Caring; Characteristics; Communities; Ensure; Female; Funding; Funding Mechanisms; Genetic; Goals; Grant; Health; Heterozygote; IACUC; Inbreeding; Income; Institution; Laboratory Animals; Macaca mulatta; Medical; Monitor; Monkeys; Population Genetics; Primates; Puerto Rico; Recovery; Regulation; Reproduction; Research; Research Personnel; Research Project Grants; Resources; SIV; Safety; Sales; Science; Services; Site Visit; Solid; Stratification; Testing; Time; United States National Institutes of Health; Universities; Viral; Virus; Visit; Work; animal care; animal colony; base; cohort; cost; genetic analysis; germ free condition; male; offspring; operation; programs; virology

PROJECT SUMMARY/ABSTRACT: The major goal of this application is to sustain the Specific Pathogen Free (SPF) program at the Caribbean Primate Research Center's (CPRC) through support of operations, administration, veterinary care, virological and MHC typing and by the improvement of our comprehensive genetic management plan. A unique feature of CPRC animals in favoring a successful establishment and sustainable of SPF colony are that these animals are naturally free of SIV and SRV-D, two of the four viruses to be tested for. Nevertheless, for safety and as required for the SPF Program, we continue systematically testing for all four viruses (B virus, STLV, SIV and SRV-D). The Program provides unadmixed Indian-origin rhesus monkeys with defined genetic and virological background. The population genetic analyses revealed that the SPF colony has remained genetically homogenous over time with sufficient amounts of heterozygosity and minimal stratification from its non-SPF founders. We plan to attain the following specific aims during the execution of the proposed plan in this Core: Aim 1: To maintain the closed CPRC- SPF indian-origin rhesus macaques colony, at a steady state level, that can provide high quality animals to support numerous NIH funded research projects, and to continue working toward self- sufficiency. The SPF colony will be maintained in compliance with the regulatory bodies. Aim 2. To use the projected recovery cost income, as part of an integral financial plan, to support the CPRC-SPF colony. With continued NIH support, the Institutional contribution and with funds from the program income, the SPF program at CPRC will remain a significant national research resource and will be able to provide healthy SPF indian-origin rhesus monkeys of defined genetic and virological background to NIH-sponsored research programs.

项目摘要/摘要： 该应用的主要目的是维持加勒比海的特定病原体（SPF）计划 灵长类动物研究中心（CPRC）通过支持操作，管理，兽医，病毒学 和MHC打字，并通过改进我们的全面遗传管理计划。一个独特的功能 CPRC动物有利于成功建立和可持续的SPF殖民地的动物是这些动物是 自然没有SIV和SRV-D，这是要测试的四种病毒中的两种。然而，出于安全和要求 对于SPF计划，我们继续系统地测试所有四种病毒（B病毒，STLV，SIV和SRV-D）。这 计划提供具有固定的遗传和病毒学背景的无混合的印度王朝恒河猴子。 人口遗传分析表明，随着时间的流逝，SPF菌落在遗传上保持均匀 其非SPF创始人的足够杂合性和最小的分层。 我们计划在此核心执行拟议计划期间实现以下特定目标：目标1： 保持稳定状态的封闭的CPRC-SPF印度恒河猕猴菌落可以提供 高质量的动物支持众多NIH资助的研究项目，并继续努力自我 足够。 SPF菌落将符合监管机构。目标2。使用 预计恢复成本收入是一项不可或缺的财务计划的一部分，以支持CPRC-SPF殖民地。 在NIH的持续支持，机构贡献和计划收入的资金下，SPF CPRC的计划将仍然是重要的国家研究资源，并将能够提供健康的SPF NIH赞助研究的定义遗传和病毒学背景的印度 - 原始恒河猴 程序。