Husbandry and Management

畜牧与管理

• 批准号: 10217278
• 负责人: Melween I. Martinez
• 金额: $ 252.45万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10217278
• 关键词: Accreditation; Acquired Immunodeficiency Syndrome; Alleles; Animal Testing; Animals; Biomedical Research; Breeding; Budgets; Caribbean region; Caring; Characteristics; Communities; Ensure; Female; Funding; Funding Mechanisms; Genetic; Goals; Grant; Health; Heterozygote; IACUC; Inbreeding; Income; Institution; Laboratory Animals; Macaca mulatta; Medical; Monitor; Monkeys; Population Genetics; Primates; Puerto Rico; Recovery; Regulation; Reproduction; Research; Research Personnel; Research Project Grants; Resources; SIV; Safety; Sales; Science; Services; Site Visit; Solid; Stratification; Testing; Time; United States National Institutes of Health; Universities; Viral; Virus; Visit; Work; animal care; animal colony; base; cohort; cost; genetic analysis; germ free condition; male; offspring; operation; programs; virology

PROJECT SUMMARY/ABSTRACT: The major goal of this application is to sustain the Specific Pathogen Free (SPF) program at the Caribbean Primate Research Center's (CPRC) through support of operations, administration, veterinary care, virological and MHC typing and by the improvement of our comprehensive genetic management plan. A unique feature of CPRC animals in favoring a successful establishment and sustainable of SPF colony are that these animals are naturally free of SIV and SRV-D, two of the four viruses to be tested for. Nevertheless, for safety and as required for the SPF Program, we continue systematically testing for all four viruses (B virus, STLV, SIV and SRV-D). The Program provides unadmixed Indian-origin rhesus monkeys with defined genetic and virological background. The population genetic analyses revealed that the SPF colony has remained genetically homogenous over time with sufficient amounts of heterozygosity and minimal stratification from its non-SPF founders. We plan to attain the following specific aims during the execution of the proposed plan in this Core: Aim 1: To maintain the closed CPRC- SPF indian-origin rhesus macaques colony, at a steady state level, that can provide high quality animals to support numerous NIH funded research projects, and to continue working toward self- sufficiency. The SPF colony will be maintained in compliance with the regulatory bodies. Aim 2. To use the projected recovery cost income, as part of an integral financial plan, to support the CPRC-SPF colony. With continued NIH support, the Institutional contribution and with funds from the program income, the SPF program at CPRC will remain a significant national research resource and will be able to provide healthy SPF indian-origin rhesus monkeys of defined genetic and virological background to NIH-sponsored research programs.

项目摘要/摘要： 该应用程序的主要目标是在加勒比海地区维持无特定病原体(SPF)计划 灵长类动物研究中心(CPRC)通过运营、管理、兽医护理、病毒学支持 和MHC分型，以及我们全面遗传管理计划的改进。的一个独特功能 CPRC动物在支持SPF殖民地的成功建立和可持续方面是这些动物 自然不含SIV和SRV-D，这是四种待检测病毒中的两种。尽管如此，为了安全和按要求 对于SPF计划，我们继续对所有四种病毒(B病毒、STLV、SIV和SRV-D)进行系统测试。这个 该计划为纯印度血统的恒河猴提供明确的遗传和病毒学背景。 群体遗传分析表明，SPF群体随着时间的推移保持了遗传同质性 具有足够的杂合度和来自其非SPF创建者的最小分层。 我们计划在执行本核心拟议计划期间实现以下具体目标：目标1： 将封闭的CPRC-SPF印度原产恒河猴群体维持在一个稳定的水平，这可以提供 高质量的动物，以支持NIH资助的众多研究项目，并继续致力于自我 足够了。SPF菌落将按照监管机构的要求进行维护。目标2.使用 预计回收成本收入，作为综合财务计划的一部分，以支持中巴合作伙伴关系-SPF殖民地。 在NIH的持续支持下，机构捐款和项目收入的资金，SPF CPRC的计划将仍然是一个重要的国家研究资源，并将能够提供健康的SPF NIH赞助的研究中具有明确遗传和病毒学背景的印度源恒河猴 程序。