MAINTENANCE OF A CLOSED CPRC SPF COLONY

封闭式 CPRC SPF 群体的维护

基本信息

批准号：
10447029
负责人：
Melween I. Martinez
金额：
$ 366.8万
依托单位：
UNIVERSITY OF PUERTO RICO MED SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-07-01 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10447029
关键词：
AIDS/HIV problem Acquired Immunodeficiency Syndrome Admixture Algorithms Animals Biological Models Biomedical Research Birth Breeding Budgets Caribbean region Caring Cercopithecine Herpesvirus 1 Characteristics Chinese Collaborations Communities Development Disease Ensure Funding Genetic Genetic Variation Genomics Geographic Locations Goals Grant Guidelines Heterozygote Human Income India Institution International Intervention Island Macaca Macaca mulatta Maintenance Medical Medical center Minority Monkeys Population Population Genetics Population Sizes Primates Provider Puerto Rico Reporting Reproduction Research Research Personnel Research Project Grants Resources Retroviridae SIV Safety Science Secure Simian Retroviruses Solid Stratification Testing Time Training United States National Institutes of Health Universities Vaccines Viral Virus animal colony biomedical resource cohort driving force effective therapy genetic analysis genetic approach germ free condition higher education human disease model development offspring operation programs recruit research and development therapeutic development

项目摘要

PROJECT SUMMARY/ABSTRACT: The major goal of this application is to sustain the Specific Pathogen Free (SPF) program at the Caribbean Primate Research Center's (CPRC) through support of operations, administration, veterinary care, virological and MHC typing and by the improvement of our comprehensive genetic management plan. A unique feature of CPRC animals in favoring a successful establishment and sustainable of SPF colony are that these animals are naturally free of SIV and SRV-D, two of the four viruses to be tested for. Nevertheless, for safety and as required for the SPF Program, we continue systematically testing for all four viruses (B virus, STLV, SIV and SRV-D). The Program provides unadmixed Indian-origin rhesus monkeys with defined genetic and virological background. The population genetic analyses revealed that the SPF colony has remained genetically homogenous over time with sufficient amounts of heterozygosity and minimal stratification from its non-SPF founders. Genetic variation will be maintained in the SPF colony using the genetic management strategies described in this application. The genetic management approach will allow for enrichment of desired MHC markers in demand for biomedical research while maintaining genetic diversity. Our approach for genetic management will be concordant with the NIH's National NHP Genetics and Genomics Consortium guidelines for ensuring the long- term viability of the CPRC's SPF colony. The estimates of Indian ancestry among the SSFS SPF colony animals is also among the highest that have been calculated across several National Primate Research Centers (NPRCs) in the US. Funds are requested in this application to sustain the SPF program and to meet the proposed aims. With continued NIH support, the Institutional contribution and with funds from the program income, the SPF program at CPRC will remain a significant national research resource and will be able to provide healthy SPF indian-origin rhesus monkeys of defined genetic and virological background to NIH-sponsored research programs.

项目摘要/摘要：该应用的主要目的是维持加勒比海的特定病原体（SPF）计划灵长类动物研究中心（CPRC）通过支持操作，管理，兽医，病毒学和MHC打字，并通过改进我们的全面遗传管理计划。一个独特的功能 CPRC动物有利于成功建立和可持续的SPF殖民地的动物是这些动物是自然没有SIV和SRV-D，这是要测试的四种病毒中的两种。然而，出于安全和要求对于SPF计划，我们继续系统地测试所有四种病毒（B病毒，STLV，SIV和SRV-D）。这计划提供具有固定的遗传和病毒学背景的无混合的印度王朝恒河猴子。人口遗传分析表明，随着时间的流逝，SPF菌落在遗传上保持均匀其非SPF创始人的足够杂合性和最小的分层。遗传使用此处描述的遗传管理策略将在SPF菌落中保持差异应用。遗传管理方法将允许富集需求中所需的MHC标记进行生物医学研究，同时保持遗传多样性。我们的遗传管理方法将是与NIH的国家NHP遗传学和基因组学联盟指南一致，以确保长期 CPRC的SPF殖民地的术语生存能力。 SSFS SPF菌落动物中印度血统的估计值也是在几个国家灵长类动物研究中心（NPRC）中计算出的最高的之一在美国。在本申请中要求资金维持SPF计划并实现拟议的目标。在NIH的持续支持，机构贡献和计划收入的资金下，SPF CPRC的计划将仍然是重要的国家研究资源，并将能够提供健康的SPF NIH赞助研究的定义遗传和病毒学背景的印度 - 原始恒河猴程序。