Immune Responses Resource Core

免疫反应资源核心

• 批准号: 10460501
• 负责人: SABRA L. KLEIN
• 金额: $ 26.63万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-30 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10460501
• 关键词: Age; Antibody Response; Antigens; Attention; B-Lymphocytes; Biological Assay; Cells; Core Facility; DNA Sequencing Facility; Data; Data Analyses; Disease; Enzyme-Linked Immunosorbent Assay; Exposure to; Flow Cytometry; Fostering; Gender; Gene Expression Profiling; Genes; Genetic; Genetic Transcription; Genomics; Genotype; Goals; Gonadal Steroid Hormones; Hemagglutinin; Human; Immune; Immune response; Immunity; Immunoglobulin Class Switching; Immunoglobulin G; Immunoglobulin M; Immunoglobulin Somatic Hypermutation; Immunologic Markers; Immunologics; Infection; Influenza; Influenza vaccination; Institution; Leadership; M2 protein; Measurement; Measures; Memory B-Lymphocyte; Mus; Neuraminidase; Pathway interactions; Phenotype; Plasmablast; Principal Investigator; Procedures; Protocols documentation; Reproducibility; Research; Research Personnel; Research Project Grants; Resources; Serology; Services; Sex Differences; Structure of germinal center of lymph node; Vaccination; Vaccine Antigen; Variant; Viral Antigens; Viral Proteins; age difference; deep sequencing; frailty; genomic data; immune function; improved; inflammatory marker; influenza virus vaccine; influenzavirus; interest; programs; response; sex; vaccine-induced antibodies

SEX AND AGE DIFFERENCES IN IMMUNITY TO INFLUENZA (SADII) IMMUNE RESPONSES RESOURCE CORE SUMMARY Under the leadership of Dr. Patricia Gearhart (Director) and Dr. Sabra Klein (Co-Director), the Immune Responses Core will provide the serological, cellular, and genetic assays that will be necessary for the accurate, and consistent measurement of sex and aged differences in immune responses to influenza vaccines and viral antigens. The Core will provide serological assessment of antibody responses to influenza vaccine and virus antigens for Research Projects 1, 2, and 3. The serological assessments will include microneutralization assays, hemagglutinin (HA) inhibition assays, and ELISAs to detect IgG that is specific for diverse influenza virus proteins, including HA, neuraminidase (NA), and the M2 protein as well as IgM and IgG isotypes to evaluate somatic hypermutation and class switching. The Core will also be responsible for conducting cellular analyses of antigen-specific (i.e., HA, NA, M2) B cells in humans and mice by flow cytometry using the MMI BD Immune Function Core facility. Particular attention will be paid to the quantification of plasmablasts and germinal center cells, including memory B cells and the recently characterized Age-associated B cells (ABCs). Finally, using the MMI Genomics and Analysis Sequencing Core, we will analyze the transcriptional variation associated with sex and age by transcriptional profiling antigen-specific B cells (i.e., plasmablasts) in humans and mice (Projects 1, 2, and 3). The transcriptional analyses will include using deep sequencing to quantify transcriptional activity in plasmablasts to evaluate the pathways differentially regulated by age and sex. Following repeated exposures to influenza antigens through infection and vaccination, an immune repertoire develops that reflects clonal selection during the primary response and recall and somatic rearrangement of VH genes following subsequent exposures, including following vaccination. V gene repertoires will be compared and analyzed for sex and age associated differences following vaccination. Together, the SADII Immune Responses Core will provide an in-depth analysis of how sex, age, and frailty alter antibody responses, B cell phenotypes, and B cell genotypes in response to influenza vaccination. With serological, cellular, and genomic assay capabilities, the SADII Immune Responses Resource Core can provide services to investigators interested in characterizing influenza virus-specific immune responses, which is currently not available at Johns Hopkins, and to SCOREs at other institutions that are seeking to measure inflammatory and immune markers of diverse diseases, beyond influenza.

性别和年龄对流感免疫反应的差异