CORE IMMUN. LAB FOR ASSESS. OF AIDS VACCINES IN PRIMATES

SIV和HIV疫苗在灵长类动物中的保护作用的证明， 导致人们对预防艾滋病的疫苗持谨慎的乐观态度。 到 将灵长类动物的发现转化为有效的艾滋病毒疫苗， 人类，了解免疫反应提供了什么是至关重要的 保护和针对抗原的免疫。 然而，在这方面， 评估灵长类动物的免疫应答是困难的， 灵长类动物中心通常没有专业知识， 动物资源分布。 例如，MHC（主要组织相容性） 复杂）分型是评价细胞毒性T淋巴细胞的必要条件 (CTL)反应，但试剂和专门知识有限。 标准 用于评估细胞介导的免疫应答的方案仍在 开发 畜用原种病毒中和效果分析 挑战是有问题的，实验室之间的测定方法不同。 此外，这些评估既费时又费力。 将资源集中在一个集中的站点有望推动该领域的发展 快速，提供必要的数据来比较免疫反应， 不同的疫苗可以产生，并将它们与保护联系起来。 三个NIAID合同支持的SIV疫苗评估单位（SIV VEU）是 目前参与SIV疫苗评估的有：杜兰大学 三角洲地区灵长类研究中心，华盛顿地区灵长类 研究中心和TSI梅森研究所。 此外，本发明还提供了一种方法， 接触数量有限的黑猩猩是通过一项 与国家癌症研究所（NCI）的机构间协议。 的 黑猩猩被安置在新墨西哥州的NCI设施中 大学灵长类中心。 这些协议只提供有限的 免疫学评价 灵长类核心免疫学实验室 合同将适应，标准化，并执行细胞和 免疫灵长类动物诱导的体液免疫应答 艾滋病疫苗原型在SIV VEU、黑猩猩单位和 其他临床前艾滋病疫苗试验，例如由 国家癌症研究所或美国陆军如果需要的话

The demonstration of protection by SIV and HIV vaccines in primates has led to cautious optimism for a protective vaccine against AIDS. To translate the findings from primates to an effective HIV vaccine in people, it is essential to understand what immune responses provide protection and against which antigen(s) immunity is directed. However, assessment of immune responses in primates is difficult and requires expertise generally not available at primate centers, where the best animal resources are located. For example, MHC (major histocompatibility complex) typing is essential for evaluation of cytotoxic T lymphocyte (CTL) responses, but reagents and expertise have been limited. Standard protocols for assessment of cell-mediated immune responses are still being developed. Analysis of virus neutralization with stocks used for animal challenge is problematic and assays vary between laboratories. Furthermore, these assessments are time-consuming and labor-intensive. Focusing resources in a centralized site is expected to advance the field rapidly, providing the necessary data to compare the immune responses that different vaccines can produce and to correlate them with protection. Three NIAID contract-supported SIV Vaccine Evaluation Units (SIV VEUs) are currently involved in the SIV vaccine evaluations: the Tulane University Delta Regional Primate Research Center, the Washington Regional Primate Research Center, and the TSI Mason Research Institute. In addition, access to a limited number of chimpanzees has been funded through an Interagency Agreement with the National Cancer Institute (NCI). The chimpanzees are housed at the NCI facilities at the New Mexico State University Primate Center. These agreements provide only limited immunological evaluation. This Primate Core Immunology Laboratory contract will adapt, standardize, and perform assays for cellular and humoral immune responses induced in primates by immunization with prototype AIDS vaccines tested at the SIV VEUs, the Chimpanzee Unit, and other Preclinical AIDS Vaccine Trials, such as those being conducted by the NCI or the United States Army, if required.