STUDIES ON TOXIC POTENTIAL OF RETROVIRAL VECTORS

前慢性研究将确定什么样的暴露方案最大限度地提高 腹膜内注射后媒介感染的概率 由Maloney小鼠白血病构建的含LacZ的载体 病毒 这些研究还将评估监测方法， 载体衍生的DNA在所选组织中的存在。 对于慢性前期 研究中，新生和断奶的F344大鼠和B6C3F1小鼠都将 采用 慢性研究将评估与癌症相关的潜在致癌性。 通过将逆转录病毒载体随机插入器官的细胞DNA 和F344大鼠和B6C3F1小鼠的组织。 根据结果， 慢性前研究，慢性研究将涉及新生儿或 断奶，并将包括治疗方案确定为最 有效

The prechronic studies will determine what exposure regimen maximizes the probability of vector infection following intraperitoneal injection with a LacZ containing vector constructed from the Maloney Murine Leukemia Virus. These studies will also evaluate methods of monitoring for the presence of vector-derived DNA in selected tissues. For the prechronic studies, both neonate and weanling F344 rats and B6C3Fl mice will be used. The chronic study will evaluate the carcinogenic potential associated with random insertion of a retroviral vector into cellular DNA of organs and tissues of F344 rats and B6C3Fl mice. Based on results of the prechronic studies, the chronic study will involve either neonates or weanlings and will include the treatment regimen determined to be most effective.