FUNCTION OF DESMOGLEIN1/PEMPHIGUS FOLIACEUS ANTIGEN
FUNCTION OF DESMOGLEIN1/PEMPHIGUS FOLIACEUS ANTIGEN
批准号:
2406206
负责人:
Kathleen Janee Green
金额:
$21.29万
依托单位国家:
美国
项目类别:
财政年份:
1993
资助国家:
美国
项目状态:
已结题
起止时间:
1993-08-01 至 2002-07-31
中文摘要
患有水疱性疾病的叶状天疱疮(PF)患者有
英文摘要
Patients with the blistering disease pemphigus foliaceus (PF) have
circulating autoantibodies whose antigenic target is a transmembrane
desmosomal cadherin called desmoglein 1 (Dsg1). It has been
hypothesized that PF IgG might directly inhibit cell adhesion by
binding to the extracellular domain of Dsg1 antibodies directly
cause PF blisters, nor had Dsg1's role in adhesion been
investigated. Over the last funding period substantial progress has
been made to define specific epitopes against which PF antibodies
are directed, to determine that binding of PF antibodies to these
epitopes is dependent on their correct conformation and to
demonstrate that anti-Dsg1 antibodies are pathogenic in the disease.
However, the precise mechanism by which anti-Dsg1 results in
acantholysis is not known. Elucidating this process will depend on
first understanding how the desmosomal cadherin adhesive complex
is assembled, maintained and regulated. Our aims for the next five
years are: 1. To define the contribution of Dsg1 and other
desmosomal cadherins to cell-cell adhesion in a fibroblast
reconstitution system and to test the hypothesis that the cytoskeletal
linker, desmoplakin, is required for desmosomal cadherin-mediated
adhesion. 2. To establish the organization of the desmosomal
cadherin extracellular domains at the adhesive interface of
desmosomes by cross-linking keratinocyte cell surface molecules
and analyzing cross-links by peptide sequencing. 3. To investigate
the basis and biological significance of the unusual 6:1
stoichiometry exhibited by the plakoglobin:Dsg1 complex and
whether plakoglobin:Dsg1 interactions are regulated by
phosphorylation of the Dsg1 cytoplasmic tail, and 4. To examine
the consequences of ectopically expressing Dsg1 or dominant
negative plakoglobin mutants on adhesion, stratification and
keratinocyte differention in organotypic raft cultures.
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Role of Desmoglein 1 in Keratinocyte-Melanocyte Communication and Melanoma
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批准号:10092121
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项目类别:
-
资助金额:$39.92万
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财政年份:2019
-
负责人:Kathleen Janee Green
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依托单位:
Role of Desmoglein 1 in Keratinocyte-Melanocyte Communication and Melanoma
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批准号:10337049
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项目类别:
-
资助金额:$38.62万
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财政年份:2019
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负责人:Kathleen Janee Green
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依托单位:
Core B STEM
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批准号:10700041
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项目类别:
-
资助金额:$20.7万
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财政年份:2019
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负责人:Kathleen Janee Green
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依托单位:
Core B STEM
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批准号:10455748
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项目类别:
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资助金额:$21.25万
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财政年份:2019
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负责人:Kathleen Janee Green
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依托单位:
Core B STEM
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批准号:10259797
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项目类别:
-
资助金额:$22.13万
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财政年份:2019
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负责人:Kathleen Janee Green
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依托单位:
Role of Desmoglein 1 in Keratinocyte-Melanocyte Communication and Melanoma
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批准号:10558743
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项目类别:
-
资助金额:$38.48万
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财政年份:2019
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负责人:Kathleen Janee Green
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依托单位:
Function of Desmoglein 1/Pemphigus Foliaceus Antigen
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批准号:7809799
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项目类别:
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资助金额:$46.43万
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财政年份:2009
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负责人:Kathleen Janee Green
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依托单位:
Keratinocyte Core
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批准号:7677671
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项目类别:
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资助金额:$18.93万
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财政年份:2009
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负责人:Kathleen Janee Green
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依托单位:
Regulation of Desmosomal Cadherins in Oral Cancer
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批准号:7805576
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项目类别:
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资助金额:$26.03万
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财政年份:2006
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负责人:Kathleen Janee Green
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依托单位:
Regulation of Desmosomal Cadherins in Oral Cancer
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批准号:7129708
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项目类别:
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资助金额:$26.76万
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财政年份:2006
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负责人:Kathleen Janee Green
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依托单位:
Regulation of Desmosomal Cadherins in Oral Cancer
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批准号:7619471
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项目类别:
-
资助金额:$26.03万
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财政年份:2006
-
负责人:Kathleen Janee Green
-
依托单位:
Regulation of Desmosomal Cadherins in Oral Cancer
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批准号:8543650
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项目类别:
-
资助金额:$24.42万
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财政年份:2006
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负责人:Kathleen Janee Green
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依托单位:
Regulation of Desmosomal Cadherins in Oral Cancer
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批准号:8295840
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项目类别:
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资助金额:$25.98万
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财政年份:2006
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负责人:Kathleen Janee Green
-
依托单位:
Regulation of Desmosomal Cadherins in Oral Cancer
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批准号:7423987
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项目类别:
-
资助金额:$26.03万
-
财政年份:2006
-
负责人:Kathleen Janee Green
-
依托单位:
Regulation of Desmosomal Cadherins in Oral Cancer
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批准号:8704400
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项目类别:
-
资助金额:$25.2万
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财政年份:2006
-
负责人:Kathleen Janee Green
-
依托单位:
Regulation of Desmosomal Cadherins in Oral Cancer
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批准号:7254711
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项目类别:
-
资助金额:$26.03万
-
财政年份:2006
-
负责人:Kathleen Janee Green
-
依托单位:
Regulation of Desmosomal Cadherins in Oral Cancer
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批准号:8895271
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项目类别:
-
资助金额:$25.98万
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财政年份:2006
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负责人:Kathleen Janee Green
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依托单位:
Epithelial Differentiation and Keratinization Gordon Con
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批准号:6669951
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项目类别:
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资助金额:$2.0万
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财政年份:2003
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负责人:Kathleen Janee Green
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依托单位:
CELL/CELL JUNCTION, STRUCTURE AND DYNAMICS IN ORAL EPITHELIUM
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批准号:6323349
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项目类别:
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资助金额:$19.43万
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财政年份:2000
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负责人:Kathleen Janee Green
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依托单位:
CELL/CELL JUNCTION, STRUCTURE AND DYNAMICS IN ORAL EPITHELIUM
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批准号:6324665
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项目类别:
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资助金额:$22.13万
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财政年份:2000
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负责人:Kathleen Janee Green
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依托单位:
海外基金