METHODS FOR DETECTION OF BK VIRUS IN ALLOGENEIC BONE MARROW TRANSPLANT

人类多瘤病毒BK病毒感染高比例的 一般人群，初发后仍潜伏在肾脏 感染。当T细胞功能处于 有缺陷的(例如，骨髓或器官移植的接受者)。 BK病毒尿也被认为是出血的原因之一 骨髓移植患者的膀胱炎。快速检测 活跃的BK病毒可能有助于对这些疾病的医疗管理 病人。 建立了一种检测BK病毒的72小时贝壳培养法 评价BK病毒在骨骼中的发生率及意义 骨髓移植患者群体。一种聚合酶的研制 BK病毒的链式反应(PCR)检测正在进行中， 并进行临床比较，以确定 贝壳小瓶培养、聚合酶链式反应或脱落细胞学检查 尿上皮细胞是最敏感、最快速的方法 正在检测BK病毒。

The human polyomavirus BK virus infects a high proportion of the general population and remains latent in the kidney after primary infection. Reactivation can occur when T cell functions are deficient (e.g., in recipients of bone marrow or organ transplants). BK viruria has also been implicated as a cause of hemorrhagic cystitis in bone marrow transplant patients. Rapid detection of active BK virus may help in the medical management of these patients. A 72-hour shell vial culture assay for BK virus was developed to evaluate the incidence and significance of BK virus in the bone marrow transplant patient population. Development of a polymerase chain reaction (PCR) assay for detection of BK virus is under way, and a clinical comparison will be performed to determine whether shell vial culture, PCR, or cytological examination of exfoliated urinary epithelial cells is the most sensitive and rapid method for detecting BK virus.