DETERMINATION OF CROSS-REACTIVITY OF A CRYPTOCOCCAL ANTIGEN EIA

检测血清或脑脊液中的隐球菌抗原可用于 隐球菌感染相关脑膜炎的诊断 新型隐球菌。 市售乳胶凝集测试套件 隐球菌抗原的检测已证明与 脱水收缩液、类风湿因子和酵母毛孢菌， 导致检测结果出现假阳性。 一种新的酶免疫分析（EIA） 套件（Premier，由 Meridan Diagnostics，辛辛那提，俄亥俄州制造） 隐球菌抗原测定现已可用，早期研究 表明其与乳胶测试的可比性。 目前该环评 与乳胶测试同时进行评估以确定其交叉 与具有高类风湿滴度的标本的反应性、标本 含有 Beigelii 毛孢子菌抗原和含有 Synersis 的样品 流体。

Detection of crytococcal antigens in serum or CSF is useful in the diagnosis of meningitis associated with infection by Cryptococcus neoformans. Commercially available latex agglutination test kits for the detection of Cryptococcal antigen have demonstrated cross-reactivity with syneresis fluid, rheumatoid factor, and the yeast Trichoporon beigelii, resulting in false positive test results. A new enzyme immunoassay (EIA) kit (Premier, manufactured by Meridan Diagnostics, Cincinnati,OH) for cryptococcal antigen determination is now available, and early studies indicate its comparability to the latex test. This EIA is currently being evaluated in parallel with the latex test to determine its cross- reactivity with specimens having high rheumatoid titers, specimens containing Trichosporon beigelii antigen, and samples containing synersis fluid.