DETERMINATION OF CROSS-REACTIVITY OF A CRYPTOCOCCAL ANTIGEN EIA

血清或脑脊液中隐球菌抗原的检测在 隐球菌致脑膜炎的诊断 新形态主义者。市售乳胶凝集试验试剂盒 对隐球菌抗原的检测显示出与 脱水液、类风湿因子和酵母菌毕氏毛滴虫， 导致假阳性检测结果。一种新的酶免疫分析方法(EIA) 套件(Premier，由俄亥俄州辛辛那提的Meridan Diagnostics制造) 隐球菌抗原测定现已可用，早期研究 表明其与乳胶试验的可比性。这项环评目前正在进行中 与乳胶试验同时进行评估，以确定其交叉- 与类风湿滴度高的标本的反应性 含有贝氏毛孢子虫抗原的样品和含有增效剂的样品 流体。

Detection of crytococcal antigens in serum or CSF is useful in the diagnosis of meningitis associated with infection by Cryptococcus neoformans. Commercially available latex agglutination test kits for the detection of Cryptococcal antigen have demonstrated cross-reactivity with syneresis fluid, rheumatoid factor, and the yeast Trichoporon beigelii, resulting in false positive test results. A new enzyme immunoassay (EIA) kit (Premier, manufactured by Meridan Diagnostics, Cincinnati,OH) for cryptococcal antigen determination is now available, and early studies indicate its comparability to the latex test. This EIA is currently being evaluated in parallel with the latex test to determine its cross- reactivity with specimens having high rheumatoid titers, specimens containing Trichosporon beigelii antigen, and samples containing synersis fluid.