DETERMINATION OF CROSS-REACTIVITY OF A CRYPTOCOCCAL ANTIGEN EIA

隐球菌抗原 EIA 交叉反应性的测定

• 批准号: 3853134
• 负责人: H D ENGLER
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3853134
• 关键词: Cryptococcus neoformans; body fluids; cerebrospinal fluid; diagnosis design /evaluation; diagnosis quality /standard; fungal antigens; human tissue; immunologic assay /test; meningitis; rheumatoid factor; yeasts

Detection of cryptococcal antigens in serum or CSF is useful in the diagnosis of meningitis associated with infection by Cryptococcus neoformans. Commercially available latex agglutination test kits for the detection of Cryptococcal antigen have demonstrated cross-reactivity with syneresis fluid, rheumatoid factor, and the yeast Trichosporon beigelii, resulting in false positive test results. A new enzyme immunoassay (EIA) kit (Premier, manufactured by Meridian Diagnostics, Cincinnati, OH) for cryptococcal antigen determination is now available, and early studies indicate its comparability to the latex test. This EIA is currently being evaluated in parallel with the latex test to determine its cross-reactivity with specimens having high rheumatoid titers, specimens containing Trichosporon beigelii antigen, and samples containing syneresis fluid.

血清或CSF中隐球菌抗原的检测可用于 隐球菌感染相关性脑膜炎的诊断 新人类 市售乳胶凝集试验试剂盒， 隐球菌抗原的检测已经证明了与 脱水收缩液、类风湿因子、酵母毕氏丝孢酵母， 导致假阳性测试结果。 一种新的酶免疫分析法 试剂盒（Premier，由Meridian Diagnostics，辛辛那提，OH制造），用于 隐球菌抗原测定现已可用，早期研究 表明其与乳胶试验的可比性。 目前，EIA正在 与乳胶试验平行评价，以确定其交叉反应性 对于具有高类风湿滴度的样本，样本含有 贝氏丝孢酵母抗原和含有脱水收缩液的样品。