EFFECT OF CENTRIFUGING SHELL VIALS AT 3500XG ON THE DETECTION OF VIRUSES

3500XG 离心壳瓶对病毒检测的影响

• 批准号: 3774523
• 负责人: H D ENGLER
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3774523
• 关键词: centrifugation; communicable disease diagnosis; diagnosis design /evaluation; diagnostic tests; human tissue; microorganism culture; virus classification

The development of the shell vial (SV) assay has greatly decreased the time to laboratory detection of many viruses in clinical specimens. Optimal conditions for the assay have been reported to be low-speed centrifugation of SV cultures, we examine whether an increased g-force of 3500xg and concomitant decreased spin time of 15 min. effect detection of viruses by the SV assay, potential specimen toxicity to the cell monolayer, and integrity of the cell monolayer. Over 300 clinical specimens will be collected and divided into 2 groups. One group will be spun at the higher speed and one group at the lower speed, and a comparison will be made of the culture results by each of the methods.

壳瓶（SV）检测的发展大大降低了 实验室检测临床标本中多种病毒的时间。 据报道，测定的最佳条件是低速 离心SV培养物，我们检查增加的g力是否 3500xg的旋转速度，同时旋转时间减少15分钟。 SV测定的病毒含量、样本对细胞的潜在毒性 单层和细胞单层的完整性。 超过300个临床 将收集样本并分为两组。 一组将 以较高的速度旋转，一组以较低的速度旋转， 将对每种方法的培养结果进行比较。