METHODS FOR RAPID DETECTION OF CYTOMEGALOVIRUS IN BLOOD SAMPLES

巨细胞病毒(CMV)是引起人类疾病的主要原因之一。 免疫受损的宿主，尤其是那些经历了实质器官移植的人 或者骨髓移植。一种简便、灵敏的早期诊断方法 检测这些患者的巨细胞病毒血液感染将有助于 病人的医疗管理。将努力确定 用pp65下基质检测血液中巨细胞病毒抗原的研究 多形核白细胞中巨细胞病毒的结构蛋白。检测 具有单抗的抗原可作为早期预测指标。 巨细胞病毒感染的可能性。比较敏感度、特异度、速度和 用贝壳小瓶病毒可以很容易地确定CMV抗原血症 培养，并用聚合酶链式反应分析立即 用于引物识别的CMV早期基因区域。

Cytomegalovirus (CMV) is a major cause of morbidity and mortality in the immunocompromised host, especially in those having undergone solid organ or bone marrow transplants. A simple and sensitive method for the early detection of CMV blood infection in these patients would be helpful for the medical management of the patient. Efforts will be made to determine the presence of CMV antigen in blood by detecting the pp65 lower matrix structural protein of CMV in polymorphonuclear leukocytes. Detection of the antigen with monoclonal antibodies may be useful as an early predictor of CMV infection. Comparisons of the sensitivity, specificity, speed, and ease of determining CMV antigenemia will be made with the shell vial viral culture and with a polymerase chain reaction assay utilizing the immediate early gene region of CMV for primer recognition.