METHODS FOR RAPID DETECTION OF CMV

这个正在进行的项目涉及几项研究。努力增加 壳小瓶法检测巨细胞病毒的敏感性 继续。我们正在研究胸腺嘧啶核苷的作用 类似物，如吲哚或溴脱氧尿苷，对CMV生长的影响 如果它们能加入我们的贝壳小瓶培养中以提高敏感性 检测临床标本中巨细胞病毒的培养。己二甲基 溴化物，或聚乙二烯，已被证明有助于人类疱疹病毒6 穿透培养中的细胞，因此增加了对 化验。我们将研究聚乙二烯对贝壳瓶中CMV的影响。 培养，看看它是否可以增加贝壳小瓶的敏感性 化验。 已发现CMV的早期结构蛋白pp65的检测 是一种快速、灵敏的预测CMV疾病进展的方法。 我们正在检测和评估一批pp65的单抗。 识别在感染病毒的细胞核中表达的表位 外周血多形核白细胞和单核细胞 与我们目前使用的pp65抗体不同。人们希望， 这些新的抗体将提高对这种情况的检测灵敏度 临床血标本中的巨细胞病毒抗原。 巨细胞病毒可能是HIV感染者肺炎的原因之一。检测 在由支气管肺泡灌洗液中发现的细胞组成的涂片中的CMV可以 一种快速诊断巨细胞病毒肺部疾病的方法。我们会研究这些 应用免疫荧光试剂涂片检测巨细胞病毒pp65和 巨细胞病毒72kd即刻早期抗原的临床应用 作为快速诊断方法。

This ongoing project involves several studies. Efforts to increase the sensitivity of the shell vial assay in detecting cytomegalovirus (CMV) continue. We are in the process of examining the effect of thymidine analogues, such as indo- or bromo-deoxyuridine, on CMV growth to determine if they can be added to our shell vial cultures to enhance the sensitivity of the culture to detect CMV in clinical specimens. Hexadimethrine bromide, or polybrene, has been demonstrated to help human herpes virus 6 penetrate cells in culture and therefore increase the sensitivity of the assay. We will examine the effect of polybrene on CMV in shell vial cultures to see if it can increase the sensitivity of the shell vial assay. The detection of pp65, the early structural protein of CMV, has been found to be a rapid and sensitive means of predicting CMV disease progression. We are examining and evaluating a pool of monoclonal antibodies to pp65 that recognizes the epitopes expressed in the nuclei of infected peripheral polymorphonuclear leukocytes and monocytes and that are different from our currently used antibodies to pp65. It is hoped that these new antibodies will allow an increased detection sensitivity of this CMV antigen in clinical blood specimens. CMV can be a cause of pneumonitis in the patient with HIV. Detection of CMV in smears consisting of cells found in bronchoalveolar lavages can be a rapid means of diagnosing CMV pulmonary disease. We will examine such smears using immunofluorescent reagents for the detection of CMV pp65 and the CMV 72kd immediate early antigen to determine their clinical utility as rapid diagnostic methods.