METHODS FOR DETECTION OF BK VIRUS IN ALLOGENEIC BONE MARROW TRANSPLANT

工作总结：人多瘤病毒BK病毒感染比例高 一般人群中，并保持潜伏在肾脏后，主要 感染当T细胞功能缺陷时， (e.g.,在骨髓或器官移植的接受者中）。BK病毒尿症具有 与骨髓出血性膀胱炎有关 移植患者快速检测活跃的BK病毒可能有助于 对这些病人进行医疗管理。72小时壳瓶培养试验 BK病毒的发病率和意义进行了评估， 骨髓移植患者人群中的BK病毒。发展 一种用于检测BK病毒的聚合酶链反应（PCR）检测方法正在进行中， 方法，并将进行临床比较，以确定是否 尿脱落细胞培养、PCR或细胞学检查 上皮细胞是最敏感和快速的检测方法 BK病毒。该项目将再持续一年。

Summary of Work: The human polyomavirus BK virus infects a high proportion of the general population and remains latent in the kidney after primary infection. Reactivation can occur when T-cell functions are deficient (e.g., in recipients of bone marrow or organ transplants). BK viruria has been implicated as a cause of hemorrhagic cystitis in bone marrow transplant patients. Rapid detection of active BK virus may help in the medical management of these patients. A 72-hour shell vial culture assay for BK virus was developed to evaluate the incidence and significance of BK virus in the bone marrow transplant patient population. Development of a polymerase chain reaction (PCR) assay for detection of BK virus is under way, and a clinical comparison will be performed to determine whether shell vial culture, PCR, or cytological examination of exfoliated urinary epithelial cells is the most sensitive and rapid method for the detection of BK virus. This project is continuing for another year.