USE OF SONICATION TO ENHANCE SHELL VIAL CULTURE OF SPECIMENS FOR CYTOMEGALOVIRUS

巨细胞病毒（CMV）感染是发病的常见原因， 免疫功能低下患者的死亡率，因此快速实验室 CMV的诊断势在必行。 病毒培养的瓶壳法， 取代了传统的试管培养作为检测CMV的主要手段， 由于外壳瓶能够在24-48小时内检测到阳性结果， 小时，相比之下，传统的平均10天至3周， 文化 由于CMV是一种高度细胞相关的病毒， 认为血液和尿液样本中细胞的破坏会释放出 病毒，从而提高培养效率。 本研究将 比较经超声处理的标本的培养结果， 接种壳瓶前细胞中的细胞相关病毒与 未经超声处理的标本，并将检查超声处理是否有助于 增加阳性培养结果的数量和/或减少 它需要文化变得积极。

Cytomegalovirus (CMV) infection is a common cause of morbidity and mortality in the immunocompromised patient, and as such rapid laboratory diagnosis of CMV is imperative. The shell vial method of virus culture has supplanted conventional tube culture as the primary means of detecting CMV, as the shell vial enables detection of a positive result within 24-48 hours, compared to an average of 10 days to 3 weeks for conventional culture. Because CMV is such a highly cell-associated virus, it was thought that disruption of cells in blood and urine specimens would release virus and thus enhance the efficiency of the culture. This study will compare culture results of specimens which have been sonicated to release cell associated virus from cells prior to inoculation of the shell vial vs. unsonicated specimens, and will examine whether sonication helps to increase the number of positive culture results and/or decreases the time it takes the culture to become positive.