USE OF SONICATION TO ENHANCE SHELL VIAL CULTURE OF SPECIMENS FOR CYTOMEGALOVIRUS

使用超声处理来增强巨细胞病毒样本的壳瓶培养

• 批准号: 3838004
• 负责人: H D ENGLER
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3838004
• 关键词: Herpesviridae disease; blood; communicable disease diagnosis; cytodiagnosis; cytomegalovirus; diagnosis design /evaluation; diagnosis quality /standard; human tissue; rapid diagnosis; tissue /cell culture; urine; vibration

Cytomegalovirus (CMV) infection is a common cause of morbidity and mortality in the immunocompromised patient, and as such rapid laboratory diagnosis of CMV is imperative. The shell vial method of virus culture has supplanted conventional tube culture as the primary means of detecting CMV, as the shell vial enables detection of a positive result within 24-48 hours, compared to an average of 10 days to 3 weeks for conventional culture. Because CMV is such a highly cell-associated virus, it was thought that disruption of cells in blood and urine specimens would release virus and thus enhance the efficiency of the culture. This study will compare culture results of specimens which have been sonicated to release cell associated virus from cells prior to inoculation of the shell vial vs. unsonicated specimens, and will examine whether sonication helps to increase the number of positive culture results and/or decreases the time it takes the culture to become positive.

巨细胞病毒(CMV)感染是发病和死亡的常见原因 免疫功能低下患者的死亡率，以及如此快速的实验室 巨细胞病毒的诊断势在必行。贝壳瓶培养病毒的方法有 取代传统的试管培养作为检测CMV的主要手段， 因为贝壳小瓶能够在24-48内检测到阳性结果 几个小时，相比之下，传统的 文化。因为CMV是一种高度与细胞相关的病毒，所以它 认为破坏血液和尿液样本中的细胞会释放出 病毒，从而提高培养效率。这项研究将 比较超声处理后释放的标本的培养结果 接种贝壳小瓶之前细胞中的细胞相关病毒与 未经超声处理的标本，并将检查超声处理是否有助于 增加阳性培养结果的数量和/或缩短时间 这需要文化变得积极。