Assembly and stability of human voltage-gated potassium channels

人类电压门控钾通道的组装和稳定性

• 批准号: DP150101929
• 负责人: Prof Jamie Vandenberg
• 金额: $ 29.01万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-01-01 至 2018-01-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP150101929
• 关键词: Assembly; stability; human; voltage; gated

The Kv11.1 voltage-gated potassium channel is an important regulator of cardiac function and a problem for the pharmaceutical industry due to its promiscuity with respect to drug binding. This project aims to investigate how Kv11.1 channels fold and assemble into tetramers and what stabilizes them in the cell membrane. Borrowing from insights gained from the structural analysis of G-Protein coupled receptors, the project intends to apply a novel protein stabilization strategy to facilitate the structural analysis of Kv11.1 channels. The successful completion of the project could reveal important insights into how these molecular machines work as well as enable atomic level studies of how drugs interact and bind to these channels.

Kv11.1电压门控钾通道是心脏功能的重要调节剂，并且由于其与药物结合的混杂性而成为制药工业的问题。该项目旨在研究Kv11.1通道如何折叠和组装成四聚体，以及是什么使它们在细胞膜中稳定。借鉴从G蛋白偶联受体的结构分析中获得的见解，该项目打算应用一种新的蛋白质稳定化策略来促进Kv11.1通道的结构分析。该项目的成功完成可以揭示这些分子机器如何工作的重要见解，并使药物如何相互作用和结合到这些通道的原子水平研究成为可能。