Pushing the limits of fluorescence microscopy with adaptive optics

利用自适应光学器件突破荧光显微镜的极限

• 批准号: LE180100001
• 负责人: Prof Leann Tilley
• 金额: $ 23.04万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Infrastructure, Equipment and Facilities
• 财政年份: 2018
• 资助国家: 澳大利亚
• 起止时间: 2018-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LE180100001
• 关键词: Pushing; limits; fluorescence; microscopy; adaptive

This project aims to establish an adaptive optics, super-resolution optical microscopy facility to image cellular events with the highest possible spatial resolution, in a whole cell or tissue context. Sophisticated computer-controlled deformable mirrors will be used to correct the way light is distorted as it passes through specimens, thereby overcoming aberrations found in thick and complex samples. This adaptive optics system will enable researchers to study complex behaviour of biological specimens, at the optical resolution limit in plant and animal tissues, leading to basic biology and biotechnology outcomes in biofuels, biomaterials and biomedicines.

该项目旨在建立一个自适应光学，超分辨率光学显微镜设备，以尽可能高的空间分辨率，在整个细胞或组织背景下成像细胞事件。精密的计算机控制的可变形镜将用于校正光穿过标本时的畸变，从而克服在厚的和复杂的样品中发现的畸变。这种自适应光学系统将使研究人员能够在植物和动物组织的光学分辨率限制下研究生物标本的复杂行为，从而在生物燃料、生物材料和生物医药方面取得基础生物学和生物技术成果。