CSF1 IL34 and the function of Macrophages in Avian Development

CSF1 IL34 和巨噬细胞在禽类发育中的功能

基本信息

  • 批准号:
    BB/H012559/1
  • 负责人:
  • 金额:
    $ 88.68万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2010
  • 资助国家:
    英国
  • 起止时间:
    2010 至 无数据
  • 项目状态:
    已结题

项目摘要

Macrophages are large white blood cells that are the first line of defense against pathogens, but also contribute to much of the pathology of infectious and inflammatory disease. Macrophages are also the bodies cellular waste disposal system, and they are needed for wound healing and for many aspects of normal development. Chickens are of interest because they are an economically important livestock species, they are tractable model in which to study development, and they are vectors for diseases that can affect humans including bacteria that cause food poisoning and avian influenza. This project aims to understand how the production of macrophages is controlled in birds and the function of macrophages in embryonic development. Our hypothesis is that two growth factors, macrophage colony-stimulating factor (CSF-1) and interleukin 34 (IL-34) act through a common receptor (the CSF-1 receptor) to promote the production, migration and function of macrophages in an embryo. In turn, the macrophages are needed for the normal process of organ formation and overall growth in the embryo. To address this hypothesis, we propose to make transgenic animals in which all of the macrophages are tagged with a fluorescent transgene so we can monitor when they appear and how they move about in the embryo. We will make the two growth factors as recombinant proteins, and make antibodies that prevent their actions. And finally, we will test the hypothesis by introducing the factors, or antibodies, into the developing embryo in the egg, to see whether macrophage production or location can be altered, and whether this changes the course of normal development. These are experiments that cannot be done easily in mammals, because the embryo cannot be access in the uterus. If our hypothesis is correct, we will identify candidate modulators of chicken immunity and growth, and also gain an insight into normal development that might be relevant to understanding human pregnancy and developmental defects.
巨噬细胞是巨大的白细胞,是抵御病原体的第一道防线,但也是感染性和炎症性疾病的主要病理因素。巨噬细胞也是体内细胞废物处理系统,它们是伤口愈合和正常发育的许多方面所必需的。鸡之所以引起人们的兴趣,是因为它们是一种重要的经济牲畜物种,它们是研究发育的易驯化模型,它们是可能影响人类的疾病的媒介,包括导致食物中毒和禽流感的细菌。该项目旨在了解鸟类中巨噬细胞的产生是如何控制的,以及巨噬细胞在胚胎发育中的功能。我们的假设是,两种生长因子,巨噬细胞集落刺激因子(CSF-1)和白介素34(IL-34)通过共同的受体(CSF-1受体)作用于促进胚胎中巨噬细胞的产生、迁移和功能。反过来,巨噬细胞是胚胎器官形成和整体生长的正常过程所必需的。为了解决这一假设,我们建议制造转基因动物,在其中所有的巨噬细胞都被标记上荧光转基因,这样我们就可以监测它们何时出现以及它们在胚胎中如何移动。我们将把这两种生长因子作为重组蛋白,并制造抗体来阻止它们的作用。最后,我们将通过将这些因子或抗体引入卵子中发育中的胚胎来检验这一假设,看看巨噬细胞的产生或位置是否可以改变,以及这是否会改变正常发育的过程。这些实验在哺乳动物身上是不容易完成的,因为胚胎不能在子宫中获得。如果我们的假设是正确的,我们将确定候选的鸡免疫和生长调节因子,并深入了解可能与理解人类怀孕和发育缺陷相关的正常发育。

项目成果

期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Functional annotation of the T-cell immunoglobulin mucin family in birds.
  • DOI:
    10.1111/imm.12607
  • 发表时间:
    2016-07
  • 期刊:
  • 影响因子:
    6.4
  • 作者:
    Hu T;Wu Z;Vervelde L;Rothwell L;Hume DA;Kaiser P
  • 通讯作者:
    Kaiser P
Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
  • DOI:
    10.1242/dev.105593
  • 发表时间:
    2014-08
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Balic A;Garcia-Morales C;Vervelde L;Gilhooley H;Sherman A;Garceau V;Gutowska MW;Burt DW;Kaiser P;Hume DA;Sang HM
  • 通讯作者:
    Sang HM
IL-4 directly signals tissue-resident macrophages to proliferate beyond homeostatic levels controlled by CSF-1.
  • DOI:
    10.1084/jem.20121999
  • 发表时间:
    2013-10-21
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Jenkins SJ;Ruckerl D;Thomas GD;Hewitson JP;Duncan S;Brombacher F;Maizels RM;Hume DA;Allen JE
  • 通讯作者:
    Allen JE
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David Hume其他文献

A continuum of expanders
  • DOI:
    10.4064/fm101-11-2016
  • 发表时间:
    2014-10
  • 期刊:
  • 影响因子:
    0
  • 作者:
    David Hume
  • 通讯作者:
    David Hume
Expresión específica de genes de la respuesta inflamactoria en subpoblaciones de macrófagos
炎症反应基因特异表达
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    V. Ripoll;David Hume;M. Fontanilla
  • 通讯作者:
    M. Fontanilla
A Treatise of Human Nature: Being an Attempt to introduce the experimental Method of Reasoning into Moral Subjects
  • DOI:
    10.2307/2216614
  • 发表时间:
    1972-04
  • 期刊:
  • 影响因子:
    0
  • 作者:
    David Hume
  • 通讯作者:
    David Hume
Direct embeddings of relatively hyperbolic groups with optimal $\ell^p$ compression exponent
  • DOI:
    10.1515/crelle-2013-0040
  • 发表时间:
    2011-11
  • 期刊:
  • 影响因子:
    0
  • 作者:
    David Hume
  • 通讯作者:
    David Hume
David Hume: Enquiries Concerning Human Understanding and Concerning the Principles of Morals (Third Edition)
大卫·休谟:关于人类理解和道德原则的探究(第三版)
  • DOI:
  • 发表时间:
    1975
  • 期刊:
  • 影响因子:
    0
  • 作者:
    David Hume;P. Nidditch
  • 通讯作者:
    P. Nidditch

David Hume的其他文献

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{{ truncateString('David Hume', 18)}}的其他基金

Coarse Geometry of Groups and Spaces
群和空间的粗略几何
  • 批准号:
    EP/V027360/2
  • 财政年份:
    2023
  • 资助金额:
    $ 88.68万
  • 项目类别:
    Fellowship
Coarse Geometry of Groups and Spaces
群和空间的粗略几何
  • 批准号:
    EP/V027360/1
  • 财政年份:
    2021
  • 资助金额:
    $ 88.68万
  • 项目类别:
    Fellowship
Macrophage Biology and Disease Susceptibility in Poultry
家禽巨噬细胞生物学和疾病易感性
  • 批准号:
    BB/M011925/1
  • 财政年份:
    2015
  • 资助金额:
    $ 88.68万
  • 项目类别:
    Research Grant
CSF1R in homeostasis and immunity
CSF1R 在体内平衡和免疫中的作用
  • 批准号:
    MR/M019969/1
  • 财政年份:
    2015
  • 资助金额:
    $ 88.68万
  • 项目类别:
    Research Grant
Understanding resistance and differential vaccine responses to Eimeria in the chicken - novel biomarkers and genetic control.
了解鸡对艾美耳球虫的耐药性和差异疫苗反应 - 新型生物标志物和遗传控制。
  • 批准号:
    BB/L004003/1
  • 财政年份:
    2014
  • 资助金额:
    $ 88.68万
  • 项目类别:
    Research Grant
Transcriptome analysis in Indian buffalo and the genetics of innate immunity
印度水牛的转录组分析和先天免疫的遗传学
  • 批准号:
    BB/L004623/1
  • 财政年份:
    2014
  • 资助金额:
    $ 88.68万
  • 项目类别:
    Research Grant
Functional Annotation of the Sheep Genome
绵羊基因组的功能注释
  • 批准号:
    BB/L001209/1
  • 财政年份:
    2013
  • 资助金额:
    $ 88.68万
  • 项目类别:
    Research Grant
Development of applications of CSF-1 and IL34 in livestock
CSF-1和IL34在家畜中的应用进展
  • 批准号:
    BB/I013113/1
  • 财政年份:
    2012
  • 资助金额:
    $ 88.68万
  • 项目类别:
    Research Grant
CSF1 and the control of postnatal growth and organ development in the rat
CSF1 与大鼠出生后生长和器官发育的控制
  • 批准号:
    G0901193/1
  • 财政年份:
    2010
  • 资助金额:
    $ 88.68万
  • 项目类别:
    Research Grant
Transcriptional control of macrophage function in the pig and its relationship to infectious disease susceptibility
猪巨噬细胞功能的转录控制及其与传染病易感性的关系
  • 批准号:
    BB/G004013/1
  • 财政年份:
    2009
  • 资助金额:
    $ 88.68万
  • 项目类别:
    Research Grant

相似海外基金

Neuron-Microglia Crosstalk in Development: A new role for the neuron-derived cytokine IL34 in microglial function
发育中的神经元-小胶质细胞串扰:神经元源性细胞因子 IL34 在小胶质细胞功能中的新作用
  • 批准号:
    10601226
  • 财政年份:
    2022
  • 资助金额:
    $ 88.68万
  • 项目类别:
Development of applications of CSF-1 and IL34 in livestock
CSF-1和IL34在家畜中的应用进展
  • 批准号:
    BB/I013113/1
  • 财政年份:
    2012
  • 资助金额:
    $ 88.68万
  • 项目类别:
    Research Grant
CSF1 IL34 and the function of macrophages in avian development
CSF1 IL34 和巨噬细胞在禽类发育中的功能
  • 批准号:
    BB/H012745/1
  • 财政年份:
    2010
  • 资助金额:
    $ 88.68万
  • 项目类别:
    Research Grant
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