MATURATION AND PLASTICITY IN THE OLFACTORY SYSTEM

嗅觉系统的成熟和可塑性

• 批准号: 3216626
• 负责人: PETER C BRUNJES
• 金额: $ 11.76万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3216626
• 关键词: afferent nerve; autoradiography; brain metabolism; cell differentiation; cell population study; developmental neurobiology; glia; immunocytochemistry; laboratory rat; microglia; neural plasticity; neuroanatomy; neurochemistry; newborn animals; olfactory lobe; opossums; protein biosynthesis; stainings; synapses

The experiments proposed below are designed to examine development in the olfactory bulb and its principal synaptic targets, the anterior olfactory nucleus and primary olfactory cortex. The olfactory system has been chosen for several reasons. First, it is highly organized, facilitating quantitative examinations of early development and making subtle alterations relatively easy to discern. Many interesting forms of early plasticity have been demonstrated in the system, including inductive tissue interactions and substantial sensitivity to early stimutation. Indeed early unilateral odor deprivation has been repeatedly demonstrated to result in some of the largest experience-induced changes observed in the central nervous system. As a result the region affords considerable potential for examinations of the mechanisms by which environmental factors help to specify early growth. Two groups of experiments are proposed. The first continues our quantitative examination of early olfactory bulb development in the rat which emphasizes studies of how alterations in functional activity affect bulb growth. Specifically, we will examine: patterns of protein synthesis both in normally developing and deprived bulbs to examine the early cell biology of the olfactory system changes in the development of bulb glia populations. Another set of studies examines early bulb growth in the gray, short-tailed opossum Monodelphis domestics. Monodelphis pups are born in a very immature state, and are quite accessible to early experimental manipulation allowing in depth examinations of early tissue interactions. Specific experiments include: examinations of early functional deprivation to gauge how activity influences early growth. assessment of the effects of bulb removal on the development of higher order olfactory structures. In summary, the proposed research will explore early developmental processes such as the instructive interactions which occur between tissues and the role of afferent activity in growth and differentiation.

下面提出的实验旨在检查 嗅球及其主要突触目标，前嗅觉 核和初级嗅皮层。 嗅觉系统已选定 有几个原因。第一，组织严密，便利 对早期发展进行定量检查并做出微妙的改变 变化相对容易辨别。早期有许多有趣的形式 可塑性已在系统中得到证明，包括感应组织 相互作用和对早期刺激的显着敏感性。确实早 单方面的气味剥夺已被多次证明会导致 在中央观察到的一些最大的经验引起的变化 神经系统。 因此，该地区具有巨大的潜力 检查环境因素有助于的机制 指定早期生长。 提出了两组实验。第一个继续我们的 大鼠早期嗅球发育的定量检查 它强调研究功能活动的改变如何影响 球茎生长。具体来说，我们将检查： 正常发育和发育不良时的蛋白质合成模式 用于检查嗅觉系统早期细胞生物学的灯泡 球状神经胶质细胞群发育的变化。 另一组研究检查了灰短尾鳀的早期鳞茎生长情况 负鼠 Monodelphis 家养。 Monodelphis 幼崽出生时非常不成熟 状态，并且很容易进行早期实验操作，从而允许 深入研究早期组织相互作用。具体实验 包括： 早期功能剥夺检查以评估活动情况 影响早期生长。 评估移除鳞茎对高等植物发育的影响 排列嗅觉结构。总之，拟议的研究将探索 早期发展过程，例如指导性互动 发生在组织之间以及传入活动在生长和发育中的作用 差异化。