Renewal of the 600 MHz solid-state NMR console for biological applications

更新用于生物应用的 600 MHz 固态 NMR 控制台

• 批准号: BB/T018119/1
• 负责人: Józef Lewandowski
• 金额: $ 35.53万
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FT018119%2F1
• 关键词: Renewal; 600; MHz; solid; state

Solid-state NMR is uniquely positioned to provide atomic resolution structural and dynamic information on complex biological systems that is highly complementary to other structural biology and biophysical techniques. It is used by researchers in the life and physical sciences to address complex problems in systems ranging from proteins to cells and even intact plants. This in turn leads to advancements in key areas from sustainable and resilient agriculture, safe nutritious food, new pharmaceuticals and better health to new low-carbon 'greener' energy, materials and everyday products.In this proposal we request to replace an obsolete and often malfunctioning solid-state NMR console with a new state-of-the-art console and two accompanying NMR probes in order to be able to perform a wide range of experiments not only faster but also on more challenging and complex systems and thus tackle a wider set of challenges related to the important societal issues. NMR probes exist in a variety of different specifications, notably with different rotor diameters as well as the number and tuning range of the rf channels - allowing different isotopes to be studied, e.g., hydrogen, carbon, nitrogen and fluorine - and different probes are best suited to different applications. The added value of the upgrade is that there is a number of other NMR probes acquired over time that could be used with the new console and benefit from presented by it improvements. This equipment will be used in a multiuser and multidisciplinary environment where scientists from different disciplines work together in order to maximise a chance of solving important problems that cannot be solved with a single "magic bullet" scientific approach. Another important aspect of this proposal is that it will enable us to train a new generation of scientists on a state-of-the-art equipment. PhD and postdocs, who would benefit from such training, could carry the skills and the knowledge they acquired as a result of it as independent scientists in academic or industrial setting and apply it to tackling new challenges.Finally, we also propose to make available to the wider scientific community part of the time on this specialised instrument for "taster" sessions during which they can test whether this methodology can provide answers to their scientific problems. Because of the highly specialised nature of solid-state NMR the end users would be supported by and could tap into the high-level expertise of the local scientists. If longer projects would be required, they could be pursued as collaborations with the experienced local scientists.

固态NMR具有独特的位置，可在复杂的生物系统上提供原子分辨率的结构和动态信息，这些信息与其他结构生物学和生物物理技术高度互补。研究人员在生活和物理科学中使用它来解决从蛋白质到细胞甚至完整植物的系统中的复杂问题。这反过来又导致了从可持续和韧性农业，安全的营养食品，新药物以及更好的健康状况到新的低碳“更绿色”能量，材料和日常产品的改善的关键领域的进步。在这项建议中，我们要求替换过时的，经常在固定范围内进行固定范围的固定范围，以替换有能力的NMR，以供您进行新的NMR，以备附上的NMR，以供您进行新的NMR。只有更快的速度，但也具有更具挑战性和更复杂的系统，因此应对与重要社会问题有关的一系列更广泛的挑战。 NMR探针存在于各种不同的规格中，特别是具有不同的转子直径以及RF通道的数量和调谐范围 - 允许研究不同的同位素，例如氢，碳，碳，氮和氟 - 以及不同的探针最适合于不同的应用。升级的附加值是，随着时间的推移，随着时间的推移获得了许多其他NMR探针，可以与新的控制台一起使用，并受益于IT改进的提出。该设备将用于多学科和多学科的环境中，其中来自不同学科的科学家共同起作用，以最大程度地解决难以解决的重要问题，这些问题无法通过单一的“魔力”科学方法来解决。该提案的另一个重要方面是，它将使我们能够在最先进的设备上培训新一代科学家。将从这种培训中受益的博士学位和博士学位可以携带他们作为学术或工业环境中的独立科学家而获得的技能和知识，并将其应用于应对新挑战。在本文中，我们还建议在这个专业工具上为“ Taster”的“ Taster” sessions of the Modealolation testology and test Onsportix testsolicy sesticy sesticixice worke works ossigication test sissions nery sissions of test termoliciation nery sessigical sissicy是否均可对他们的科学进行回答。由于固态NMR的高度专业化本质，最终用户将得到支持，并可以利用当地科学家的高级专业知识。如果需要更长的项目，则可以将其作为与经验丰富的当地科学家的合作。

项目成果

Elucidation of microbial lignin degradation pathways using synthetic isotope-labelled lignin.

• DOI: 10.1039/d2cb00173j
• 发表时间: 2023-01-04
• 期刊: RSC CHEMICAL BIOLOGY
• 影响因子: 4.1
• 作者: Alruwaili, Awatif;Rashid, Goran M. M.;Sodre, Victoria;Mason, James;Rehman, Zainab;Menakath, Anjali K.;Cheung, David;Brown, Steven P.;Bugg, Timothy D. H.
• 通讯作者: Bugg, Timothy D. H.