The molecular basis of how cyst nematodes coordinate their life cycle with their host

胞囊线虫如何与宿主协调其生命周期的分子基础

• 批准号: BB/V00249X/1
• 负责人: John Jones
• 金额: $ 53.14万
• 依托单位: University of St Andrews
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FV00249X%2F1
• 关键词: molecular; basis; how; cyst; nematodes

Plant-parasitic nematodes cause huge damage to global agriculture. In the UK, the potato cyst nematodes (PCN) are the most important pests of potato and an absence of effective control measures for PCN causes major problems for potato growers. A key point in the PCN life cycle is a dormant survival stage - the second stage juvenile - which remains unhatched and protected in the eggs. As PCN cannot infect other crops the unhatched nematode remains dormant until chemical cues are detected from the roots of growing potato plants. In our previous work we have identified an annexin protein present in the eggshell of PCN. We have shown that this annexin is required by the nematode to maintain the normal function of the eggshell and that the protein undergoes a change in the presence of the chemical cues present in root diffusates. These data suggest that this protein plays a key role in allowing the nematode to respond to the presence of host plants. Annexins are calcium dependent lipid binding proteins. In this project we aim to determine how the annexin responds to the presence of host chemical cues and calcium to control changes in the permeability of the lipid layers in the eggshell and thus provides a link between the nematode life cycle and the presence of a host plant.

植物寄生线虫对全球农业造成巨大损害。在英国，马铃薯孢囊线虫（PCN）是马铃薯最重要的害虫，缺乏有效的防治措施给马铃薯种植者带来了重大问题。多氯化萘生命周期的一个关键点是休眠生存阶段-第二阶段幼鱼-它在卵中未孵化并受到保护。由于多氯化萘不能感染其他作物，未孵化的线虫一直处于休眠状态，直到从生长的马铃薯植物根部检测到化学线索。在我们以前的工作中，我们已经确定了一个膜联蛋白蛋白存在于蛋壳的PCN。我们已经表明，这种膜联蛋白是所需的线虫，以维持正常的功能的蛋壳和蛋白质经历了变化的存在下，存在于根扩散的化学线索。这些数据表明，这种蛋白质在允许线虫对宿主植物的存在作出反应方面起着关键作用。膜联蛋白是钙依赖性脂质结合蛋白。在这个项目中，我们的目标是确定膜联蛋白如何响应宿主化学信号和钙的存在，以控制蛋壳中脂质层渗透性的变化，从而提供线虫生命周期和宿主植物的存在之间的联系。

项目成果

An eggshell-localised annexin plays a key role in the coordination of the life cycle of a plant-parasitic nematode with its host.

• DOI: 10.1371/journal.ppat.1011147
• 发表时间: 2023-02
• 期刊: PLoS pathogens
• 影响因子: 6.7