Bioanalytical Separation Using Chiral Polymers

使用手性聚合物进行生物分析分离

• 批准号: 6496853
• 负责人: ISIAH M WARNER
• 金额: $ 1.58万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6496853
• 关键词: analytical ultracentrifugation; capillary electrophoresis; chemical aggregate; chemical chain length; chemical synthesis; crosslink; high performance liquid chromatography; hydropathy; infrared spectrometry; interferometry; light scattering; mass spectrometry; mathematical model; micelles; molecular polarity; neutron diffraction; nuclear magnetic resonance spectroscopy; peptides; physical separation; polyglutamates; polymerization; polymers; stereoisomer; surfactant; thermodynamics

DESCRIPTION (provided by applicant) This project continues to focus on synthesis and development of new chiral reagents foruse in chiral separation methods for resolution of racemic mixtures. Four different areas of investigations are suggested for this renewal proposal. The first area involved continued studies on the use of chiral polymers as mobile phase additives to achieve chiral separations in Capillary Electrophoresis (CE) through an approach known as micellar electrokinetc chromatography (MEKC). The focus continues to be on polymeric surfactants, which are produced by photo-induced focus on properties of polymeric surfactants which continue to chiral recognition, including 1) surfactant tail length, 2) head group variations1 3) vesicle polymers as chiral reagents, and 4) other parameters such as change in counterions, pH, and ionic strength. New chiral polymers (e.g. chiral resorcinarenes will also be examined in this phase of our research. The second part of this proposed research involves the use of polymeric surfactants as stationary phases for chiral separations. It is proposed that these chiral polymers can be easily linked to silica particles and used as stationary phases to achieve separation Possible approaches to linking these polymers to silica to crea stationary phases are outlined. The application of this approach to capillary electro chromatography (CEC) opens new avenues for chiral separation. The third component of this research involves a combination of the two approaches that are proposed above, i.e. a use of chiral micelle polymers in the mobile phase in combination with chiral polumeric surfactants as stationary phases. It is reasonable to expect that this approach will produce synergistic affects in most separations, it not at all. The fourth component of our research involves a variety of approaches which have resulted from the attention and interest that our research has engendered from other scientists, primarily organic chemists. Thus, these studies will largely be collaborative in nature. These studies will involve an examination of other kinds of chiral polymers as possible separation agents, primarily in CE and CEC.

描述（由申请人提供）该项目继续专注于手性分离的新手性试剂的合成和开发 消除外星人混合物的方法。四个不同的区域 建议对该更新建议进行调查。涉及的第一个区域 继续研究手性聚合物作为流动相添加剂 通过毛细管电泳（CE）实现手性分离 方法称为胶束电动色谱法（MEKC）。重点 继续使用聚合物表面活性剂，由光诱导产生 关注继续手性的聚合物表面活性剂的特性 识别，包括1）表面活性剂尾部长度，2）头部组变化1 3） 囊泡聚合物作为手性试剂，以及4）其他参数，例如变化 柜台，pH和离子强度。新的手性聚合物（例如手性 在我们的研究的这一阶段，还将检查植菌烯。第二个 这项提出的研究的一部分涉及使用聚合物表面活性剂作为 手性分离的固定相。有人提出这些手性 聚合物可以很容易地链接到二氧化硅颗粒并用作固定相 为了实现将这些聚合物与二氧化硅联系起来的可能方法 概述了CREA固定阶段。这种方法的应用 毛细管电印（CEC）为手性分离开辟了新的途径。 这项研究的第三部分涉及两者的组合 上面提出的方法，即使用手性胶束聚合物 流动阶段与手性腐烂的表面活性剂结合在一起 阶段。可以合理地期望这种方法会产生协同作用 在大多数分离中都没有影响。我们的第四个组成部分 研究涉及各种方法，这些方法是由 我们的研究引起了其他科学家的关注和兴趣， 主要是有机化学家。因此，这些研究在很大程度上将是合作的 本质。这些研究将涉及对其他手性的检查 聚合物作为可能的分离剂，主要在CE和CEC中。