GANGLIONIC CONTROL OF MESENTERIC BLOOD VESSELS

腹椎前神经节包括腹腔神经节、上级神经节和腹侧神经节 肠系膜和肠系膜下神经节，这些神经节提供 腹部和盆腔血管的交感神经支配。 的 这些神经元的活动控制着大脑的生理状态。 内脏血管床 这些神经元的活动水平 从神经和激素输入集成;这些输入包括传出 交感神经流出，初级感觉神经元和特殊的肠和 血管感觉神经元 本研究的目的是确定 神经节的每一个输入控制和调节的机制， 肠系膜循环 这些研究的结果将 不仅对肠系膜血管床，而且对所有 神经控制起作用的血管床。 使用 电生理学、解剖学和药理学技术 研究将确定以下内容：1） 感觉抑制性连接电位（IJP）和相关的血管舒张 肠扩张引起的肠系膜动脉，包括 神经介导的反应和神经递质参与; 2） 肠系膜动脉和静脉的比较药理学，以阐明 肠系膜静脉反应性更强的潜在机制 神经激活; 3）生理和药理学特性 胆碱能蕈毒碱E.P.S.P.S.肠内生理诱发 椎前神经节神经元的生物物理特性 在分离的神经元中用膜片钳测定; 5）离子 分离的神经节细胞中毒蕈碱去极化的机制 用膜片钳和单电极电压测定神经元 钳; 6）神经节神经元中产生的去极化机制， 候选肽类神经递质P物质，血管活性肠 肽和降钙素基因相关肽; 7）表征交感神经 神经血管平滑肌相互作用，通过评估神经肌肉 神经元-平滑肌共培养物中的传递。 的结果予以 研究将提供一个基本的了解， 交感神经系统控制血管， 治疗血管和胃肠道疾病的基本原理， 具有神经原性成分，包括高血压、局部缺血， 炎症性肠病

The abdominal prevertebral ganglia consist of the celiac, superior mesenteric and inferior mesenteric ganglia and these ganglia provide the sympathetic innervation of the abdominal and pelvic blood vessels. The activity in these neurons controls the physiological state of the splanchnic vascular bed. The level of activity in these neurons is integrated from neural and hormonal inputs; these inputs include efferent sympathetic outflow, primary sensory neurons and special enteric and vascular sensory neurons. It is the aim of this research to determine the mechanisms whereby each of the inputs to ganglia control and modulate the mesenteric circulation. The findings from these studies will have implications not only for the mesenteric vascular bed but also for all vascular beds where neural control is operative. Using electrophysiological, anatomical and pharmacological techniques these studies will determine the following: 1) the characteristics of the sensory inhibitory junction potential (IJP) and associated vasodilatation evoked in mesenteric artery by intestinal distension, including which nerves mediate the response and neurotransmitter(s) involved; 2) the comparative pharmacology of mesenteric arteries and veins to elucidate the mechanisms underlying the greater responsiveness of mesenteric veins to nerve activation; 3) the physiological and pharmacological properties of cholinergic muscarinic e.p.s.p.s. evoked physiologically with intestinal distension; 4) biophysical properties of prevertebral ganglionic neurons determined with patch-voltage clamp in dissociated neurons; 5) ionic mechanism(s) of muscarinic depolarizations in dissociated ganglionic neurons determined with patch-voltage clamp and single electrode voltage clamp; 6) mechanism(s) of depolarizations produced in ganglionic neurons by candidate peptide neurotransmitters substance P, vasoactive intestinal peptide and calcitonin gene-related peptide; 7) characterize sympathetic nerve vascular smooth muscle interactions by evaluating neuromuscular transmission in neuron-smooth muscle co-cultures. The results of these studies will provide a fundamental understanding of the ways in which the sympathetic nervous system controls blood vessels and will provide rationale for the treatment of vascular and gastrointestinal disorders that have a neurogenic component including hypertension, ischemia, and inflammatory bowel disorders.