寄生虫病的生理和细胞化学病理学-猫眼课题宝

寄生虫病的生理和细胞化学病理学

批准号：

3809547

负责人：

T I MERCADO

金额：

$0.0万

依托单位：

NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES

依托单位国家：

美国

项目类别：

财政年份：

资助国家：

美国

项目状态：

未结题

起止时间：

至

来源：

https://reporter.nih.gov/project-details/3809547

关键词：

Pseudomonas Trypanosoma cruzi antibiotics antiprotozoal agents carbohydrate structure chemical structure chromatography drug design /synthesis /production drug metabolism glycosides high performance liquid chromatography histochemistry /cytochemistry mass spectrometry nuclear magnetic resonance spectroscopy parasitic disease chemotherapy protein structure trypanosomiasis

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英文摘要

Studies on the purification of a lytic, anti-trypanosomal factor from the bacterial species, Pseudomonas fluorescens, were continued. Reversed-phase high performance liquid chromatography (HPLC) was used in our attempts to obtain fractions suitable for NMR and mass spectroscopic analyses. Although a considerably purified compound was obtained, structural analysis was complicated by the occurrence of Tris in the purified fractions. This chemical was present in the defined medium used for the cultivation of the bacterium and persisted through various chromatographic column elutions. After decontamination of the samples employing several procedures for Tris removal, NMR analysis disclosed the occurrence of glycosidic linkages in this antibiotic but further decontamination will be necessary to obtain a complete structural characterization. The determination of chemical structure is essential before studies on synthesis are initiated and pharmacological and toxicological properties are examined employing Trypanosoma cruzi infections.

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