PHYSIOLOGICAL AND CYTOCHEMICAL PATHOLOGY OF PARASITIC DISEASES

一种裂解抗锥虫因子的纯化研究 细菌种类为荧光假单胞菌。反相 我们使用高效液相色谱(HPLC)来尝试 获得适合于核磁共振和质谱分析的级分。虽然 得到了纯度较高的化合物，并进行了结构分析。 在精制组分中出现Tris而变得复杂。这 化学物质存在于规定的培养基中。 细菌，并通过各种色谱柱洗脱。 在对使用TRIS的几种程序的样品进行去污之后 除去，核磁共振分析揭示了糖苷键的存在 这种抗生素但需要进一步的净化才能获得 完整的结构表征。化学物质的测定 在开始研究合成之前，结构是必不可少的 药理和毒理学特性的检查使用 克氏锥虫感染。

Studies on the purification of a lytic, anti-trypanosomal factor from the bacterial species, Pseudomonas fluorescens, were continued. Reversed-phase high performance liquid chromatography (HPLC) was used in our attempts to obtain fractions suitable for NMR and mass spectroscopic analyses. Although a considerably purified compound was obtained, structural analysis was complicated by the occurrence of Tris in the purified fractions. This chemical was present in the defined medium used for the cultivation of the bacterium and persisted through various chromatographic column elutions. After decontamination of the samples employing several procedures for Tris removal, NMR analysis disclosed the occurrence of glycosidic linkages in this antibiotic but further decontamination will be necessary to obtain a complete structural characterization. The determination of chemical structure is essential before studies on synthesis are initiated and pharmacological and toxicological properties are examined employing Trypanosoma cruzi infections.