PHYSIOLOGICAL AND CYTOCHEMICAL PATHOLOGY OF PARASITIC DISEASES

抗锥虫溶解因子的纯化研究 细菌种类荧光假单胞菌继续。反相 高效液相色谱法（HPLC）用于我们的尝试， 获得适合于NMR和质谱分析的级分。虽然 得到相当纯的化合物，结构分析如下： 由于纯化级分中出现Tris而复杂化。这 化学品存在于用于培养的确定成分培养基中。 细菌，并坚持通过各种色谱柱洗脱。 在对样品进行去污后，采用几种程序对Tris 除去后，NMR分析揭示了糖苷键的存在， 这种抗生素，但进一步净化将是必要的， 完整的结构特征。测定化学 在开始合成研究之前，结构至关重要， 药理学和毒理学性质的检查，采用 克氏锥虫感染。

Studies on the purification of a lytic, anti-trypanosomal factor from the bacterial species, Pseudomonas fluorescens, were continued. Reversed-phase high performance liquid chromatography (HPLC) was used in our attempts to obtain fractions suitable for NMR and mass spectroscopic analyses. Although a considerably purified compound was obtained, structural analysis was complicated by the occurrence of Tris in the purified fractions. This chemical was present in the defined medium used for the cultivation of the bacterium and persisted through various chromatographic column elutions. After decontamination of the samples employing several procedures for Tris removal, NMR analysis disclosed the occurrence of glycosidic linkages in this antibiotic but further decontamination will be necessary to obtain a complete structural characterization. The determination of chemical structure is essential before studies on synthesis are initiated and pharmacological and toxicological properties are examined employing Trypanosoma cruzi infections.