STUDIES ON THE ANTI-TRYPANOSOMAL FACTOR FROM PSEUDOMONAS FLUORESCENS

根据1991-92年的年度报告，我于1月2日退休， 1993年。由于Twinbrook II设施的翻新，我不得不 腾出我的实验室空间，4号楼没有空间可用来 继续这项工作。然而，在我退休期间，我一直能够 继续我与乔治·哈默博士的合作 乔治城大学的化学，这个项目的核磁共振专家。他有 鉴定了抗锥虫因子(ATF)的两个主要成分， 一种肽和一种α-D-糖苷。还有第三个组件，即 仍有待确认。为了方便在这方面的工作 化学结构的阐明，我已经获得了合作 Frank Robey博士，多肽和免疫化学组主任，实验室 细胞发育和肿瘤学研究所。我们用的是反向的- 相高效液相色谱(HPLC)试图获得 更好地分离了用于核磁共振鉴定的组分。我有过 能够进行锥虫生物检测(伊氏锥虫) 在7号楼由动物主管梅塔·蒂蒙斯博士提供的空间 NIAID动物保育科设施。 另一项与Serap博士合作的研究正在继续进行 阿尔索伊，耶鲁大学麦克阿瑟分子寄生虫学中心 康涅狄格州纽黑文医学院。这项研究的目标是克隆和 利用共生菌正常表达AFT-II基因 存在于采采蝇的肠道中，非洲的媒介 锥虫病。产ATF共生菌的重新引入 采采蝇理论上应该可以预防锥虫感染。

As reported in the annual report for 1991-92, I retired on January 2, 1993. Because of the renovation of the Twinbrook II Facility, I had to vacate my lab space and there was no space available in Building 4 to continue this work. During my retirement, however, I have been able to continue my collaboration with Dr. George Hammer, Department of Chemistry, Georgetown University, the NMR expert on this project. He has identified the two main components to the anti-trypanosomal factor (ATF), a peptide and an alpha-d-glycoside. There is a third component, which remains to be identified. In order to facilitate the work in the elucidation of the chemical structure, I have obtained the collaboration of Dr. Frank Robey, Chief, Peptide and Immunochemistry Unit, Laboratory of Cellular Development and Oncology, NIDR. We are employing reversed- phase high performance chromatography (HPLC) in an attempt to obtain a better separation of the components for the NMR identification. I have been able to perform the trypanosome bioassays (Trypanosoma Equiperdum) in space provided in Building 7 by Dr. Meta Timmons, Chief, Animal Facility, Animal Care Branch, NIAID. An additional, collaborative study is being continued with Dr. Serap Alssoy, MacArthur Center for Molecular Parasitology, Yale University School of Medicine, New Haven, CT. The goal of the study is to clone and express the gene encoding for the AFT-II employing symbionts normally present in the gut of the tsetse fly, the vector of African trypanosomiasis. Re-introduction of such an ATF-producing symbiont into tsetse flies should theoretically prevent trypanosomal infections.