寄生虫病的生理和细胞化学病理学-猫眼课题宝

寄生虫病的生理和细胞化学病理学

批准号：

3960436

负责人：

T I MERCADO

金额：

$0.0万

依托单位：

NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES

依托单位国家：

美国

项目类别：

财政年份：

资助国家：

美国

项目状态：

未结题

起止时间：

至

来源：

https://reporter.nih.gov/project-details/3960436

关键词：

Pseudomonas Trypanosoma Trypanosoma cruzi antibiotics antiprotozoal agents biological products high performance liquid chromatography histochemistry /cytochemistry microorganism culture microorganism growth microorganism interaction microorganism metabolism parasitic disease chemotherapy parasitic diseases protozoal infection protozoal toxin scanning electron microscopy

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中文摘要

翻译

一种细菌抗锥虫因子的研究荧光假单胞菌公开了吸附层析，高性能硅胶柱除去了大部分非活性组分， ATF，产生明显更纯的级分。逆流色谱法（伊藤线圈）在生产较大的更纯的物质。这两种技术都有助于去除大多数脂多糖从裂解馏分，提供了一个明确的优势，为未来的体内化疗研究，克氏锥虫

英文摘要

Studies on an anti-trypanosomal factor (ATF) from the bacterial species Pseudomonas fluorescens disclosed that adsorption chromatography with high performance silica columns removed most of the inactive components from the ATF, yielding a fraction which was markedly purer. Counter current chromatography (Ito Coil) aided significantly in the production of larger amounts of the purer substance. Both techniques were useful in removing most of the lipopolysaccharide from the lytic fractions, providing a definite advantage for future in vivo chemotherapeutic studies against Trypanosoma cruzi.

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