PHYSIOLOGICAL AND CYTOCHEMICAL PATHOLOGY OF PARASITIC DISEASES

抗锥虫因子的研究 种，荧光假单胞菌披露，逆流 使用I至多层螺旋的色谱（CCC） 柱对于较大样品的分馏非常有效 不降低分辨率或延长 分离的速度。 产生了显著的溶解活性 纯化级分的量低至80 μ g。 也是 可以分离两种类型的组件，一种是裂解 寄生虫和一种麻痹和杀死，但不溶解。 的 这些因素之间的差异进一步建立在 蛋白质测定和超微结构研究的基础 用这些处理的马疫锥虫的锥鞭毛体 馏分，并与高效液相色谱法 （HPLC）。 CCC-HPLC系统将有助于未来的研究， 这种抗生素结构和合成及其 实验性T.克鲁兹

Studies on an anti-trypanosomal factor (ATF) from the bacterial species, Pseudomonas fluorescences disclosed that countercurrent chromatography (CCC) employing the I to multilayer coiled column was very effective for the fractionation of larger samples of the crude product without decreasing resolution or prolonging the speed of separation. Significant lytic activity was produced by amounts of the purified fractions as low as 80 mug. It was also possible to separate two types of components, one that lysed the parasites and one that paralysed and killed, but did not lyse. The difference between these factors was established further on the basis of protein assays and ultrastructural studies of trypomastigotes of Trypanosoma equiperdum treated with these fractions, and with high performance liquid chromatography (HPLC). The CCC-HPLC system will aid in future research on the structure and synthesis of this antibiotic and its chemotherapeutic effect in experimental infections with T. cruzi.