ROLE OF SULFATED GLYCOCONJUGATES IN TUMOR CELL ADHESION

硫酸化糖复合物在肿瘤细胞粘附中的作用

• 批准号: 3752082
• 负责人: D D ROBERTS
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3752082
• 关键词: apolipoproteins; carbohydrate structure; cell adhesion; cell adhesion molecules; chemical binding; chemical structure function; extracellular matrix; gene expression; glycolipids; glycoproteins; heparan sulfate; human tissue; laminin; molecular site; neoplastic cell; protein structure; proteoglycan; small cell lung cancer; sulfates; thrombospondins; tissue /cell culture

We have found that sulfated glycolipids participate in tumor cell adhesion by directly promoting adhesion (Cancer Res 1988;48:3367) and as receptors for thrombospondin on melanoma cells (Cancer Res l988;48:6785). Relatively few of the glycolipids belonging to this class have been characterized. We are purifying novel sulfated glycolipids from several sources including human kidney and meconium and breast and small cell lung carcinoma cell lines. The structures of these glycolipids will be examined using chemical and immunological approaches. Monoclonal antibodies to these will be used to examine the potential of these structures as tumor markers. The molecular basis of binding of adhesive glycoproteins to sulfatide and heparan sulfate proteoglycans are being investigated by identifying sequences in these molecules responsible for binding. Both proteolytic and recombinant fragments of the adhesive proteins are being used to map the active binding domains.

我们发现硫酸化糖脂参与肿瘤细胞粘附 通过直接促进粘附（Cancer Res 1988;48：3367）和作为受体 对于黑素瘤细胞上的血小板反应蛋白（Cancer Res 1988;48：6785）。 属于这类的糖脂相对较少， 表征了 我们正在纯化新的硫酸化糖脂， 来源包括人肾、胎粪、乳腺和小细胞肺 癌细胞系。 这些糖脂的结构将是 使用化学和免疫学方法进行检查。 单克隆 这些抗体将被用来检查这些潜在的 结构作为肿瘤标志物。 胶粘剂粘接的分子基础 硫苷脂和硫酸乙酰肝素蛋白聚糖的糖蛋白正在被 通过鉴定这些分子中负责 约束力 粘合剂的蛋白水解和重组片段 蛋白质被用于绘制活性结合结构域。