ROLE OF SULFATED GLYCOCONJUGATES IN TUMOR CELL ADHESION

我们发现硫酸糖脂参与了肿瘤细胞的黏附。 通过直接促进黏附(癌症决议1988；48：3367)和作为受体 用于黑色素瘤细胞的凝血酶反应蛋白(癌症研究报告1988；48：6785)。相对的 属于这一类的糖脂很少被表征。我们 正在从几个来源提纯新型的硫酸化糖脂，包括 人肾、胎粪、乳腺和小细胞肺癌细胞 台词。这些糖脂的结构将用化学方法进行检测。 和免疫学方法。将使用针对这些的单抗。 以检验这些结构作为肿瘤标记物的潜力。这个 硫脂与粘附性糖蛋白结合的分子基础 硫酸乙酰肝素蛋白多糖正在通过鉴定 这些分子中负责结合的序列。既能分解蛋白质，又能 黏附蛋白的重组片段正被用来绘制 活性结合结构域。

We have found that sulfated glycolipids participate in tumor cell adhesion by directly promoting adhesion (Cancer Res 1988;48:3367) and as receptors for thrombospondin on melanoma cells (Cancer Res 1988;48:6785). Relatively few of the glycolipids belonging to this class have been characterized. We are purifying novel sulfated glycolipids from several sources including human kidney and meconium and breast and small cell lung carcinoma cell lines. The structures of these glycolipids will be examined using chemical and immunological approaches. Monoclonal antibodies to these will be used to examine the potential of these structures as tumor markers. The molecular basis of binding of adhesive glycoproteins to sulfatide and heparan sulfate proteoglycans are being investigated by identifying sequences in these molecules responsible for binding. Both proteolytic and recombinant fragments of the adhesive proteins are being used to map the active binding domains.