ROLE OF SULFATED GLYCOCONJUGATES IN TUMOR CELL ADHESION

我们发现硫酸化糖脂参与肿瘤细胞粘附 通过直接促进粘附（Cancer Res 1988;48：3367）和作为受体 对于黑素瘤细胞上的血小板反应蛋白（Cancer Res 1988;48：6785）。 相对 属于这一类的糖脂很少被表征。 我们 正在从几种来源纯化新型硫酸化糖脂， 人肾癌、胎粪癌、乳腺癌和小细胞肺癌细胞 线 这些糖脂的结构将使用化学方法进行检查。 和免疫学方法。 将使用针对这些的单克隆抗体 来检测这些结构作为肿瘤标志物的潜力。 的 粘附糖蛋白与硫苷脂结合的分子基础， 硫酸乙酰肝素蛋白聚糖正在通过鉴定 这些分子中负责结合的序列。 既有蛋白水解作用， 粘附蛋白的重组片段被用于绘制 活性结合域。

We have found that sulfated glycolipids participate in tumor cell adhesion by directly promoting adhesion (Cancer Res 1988;48:3367) and as receptors for thrombospondin on melanoma cells (Cancer Res 1988;48:6785). Relatively few of the glycolipids belonging to this class have been characterized. We are purifying novel sulfated glycolipids from several sources including human kidney and meconium and breast and small cell lung carcinoma cell lines. The structures of these glycolipids will be examined using chemical and immunological approaches. Monoclonal antibodies to these will be used to examine the potential of these structures as tumor markers. The molecular basis of binding of adhesive glycoproteins to sulfatide and heparan sulfate proteoglycans are being investigated by identifying sequences in these molecules responsible for binding. Both proteolytic and recombinant fragments of the adhesive proteins are being used to map the active binding domains.