GLYCOLIPID ANTIGENS EXPRESSED IN ESOPHAGEAL CARCINOMA

食管癌中表达的糖脂抗原

• 批准号: 3813430
• 负责人: D D ROBERTS
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3813430
• 关键词: China; antitumor antibody; ceramide tetrahexoside; esophagus neoplasm; fucose; galactosamine; glycolipids; human subject; human tissue; monoclonal antibody; neoplasm /cancer immunodiagnosis; sialate; surface antigens; tissue /cell culture; tumor antigens

Esophageal carcinoma is endemic in the Ho Nam Province in China. Characterization of antigens unique to these tumors would be useful for diagnosis of this carcinoma. Glycolipids isolated from esophageal tissues obtained from 11 cancer patients, 10 normal individual controls, and a cultured esophageal carcinoma line were examined using several monoclonal antibodies. Several glycolipids differ in amount between the tumor samples and normal controls. Both neutral and sialylated glycolipids containing 3-fucosyllactosamine sequences differ in expression between the tumor and control tissues and a heptaglycosylceramide reacting with antibodies to this sequence is found only in the tumor tissues. The same glycolipids are detected in the cultured cell line, confirming that the tumor antigen is produced by the tumor and is not due to contamination by infiltrating granulocytes.

食管癌是中国河南省的地方病。 这些肿瘤特有的抗原的表征将有助于 诊断这种癌症。从食管组织中分离的糖脂 从11名癌症患者、10名正常对照个体和一名 用几种单克隆抗体检测培养的食管癌细胞系， 抗体的几种糖脂在肿瘤样品之间的量不同 和正常对照。中性糖脂和唾液酸化糖脂均含有 3-岩藻糖基乳糖胺序列在肿瘤之间的表达不同， 对照组织和与抗体反应的七糖基神经酰胺， 该序列仅在肿瘤组织中发现。同样的糖脂 在培养的细胞系中检测到，证实肿瘤抗原是 由肿瘤产生，而不是由于浸润污染 粒细胞