ROLE OF SULFATED GLYCOCONJUGATES IN TUMOR CELL ADHESION

我们发现硫酸糖脂参与了肿瘤细胞的黏附。 通过直接促进粘连(癌症研究48：3367,1988)和AS 黑色素瘤细胞上的凝血酶反应蛋白受体(癌症研究报告48：6785， (1988年)。属于这一类的糖脂中相对较少的是 特色化的。我们正在从几个新的硫化糖脂中提纯 来源包括人肾和胎粪、乳房和小细胞肺 癌细胞系。我们将研究这些糖脂的结构。 使用化学和免疫学方法。抗这些的单抗 将被用来研究这些结构作为肿瘤标志物的潜力。 黏附糖蛋白与硫脂结合的分子基础是 通过识别这些分子中的序列进行研究 用于装订。为了进一步表征硫化糖脂对 肿瘤细胞的行为，我们也在研究细胞内的反应 黑色素瘤细胞附着在硫脂涂层的底物上。

We have found that sulfated glycolipids participate in tumor cell adhesion by directly promoting adhesion (Cancer Res. 48: 3367, 1988) and as receptors for thrombospondin on melanoma cells (Cancer Res. 48: 6785, 1988). Relatively few of the glycolipids belonging to this class have been characterized. We are purifying novel sulfated glycolipids from several sources including human kidney and meconium and breast and small cell lung carcinoma cell lines. The structures of these glycolipids will be examined using chemical and immunological approaches. Monoclonal antibodies to these will be used to examine the potential of these structures as tumor markers. The molecular basis of binding of adhesive glycoproteins to sulfatide is being investigated by identifying sequences in these molecules responsible for binding. To further characterize the effects of sulfated glycolipids on tumor cell behavior, we are also examining the intracellular responses following attachment of melanoma cells on sulfatide-coated substrates.