GLYCOCONJUGATE ANTIGENS EXPRESSED IN CANCER CELLS

癌细胞中表达的糖结合抗原

• 批准号: 3774376
• 负责人: D D ROBERTS
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3774376
• 关键词: adenocarcinoma; biomarker; breast neoplasms; carbohydrate structure; colon neoplasms; glycolipids; glycoproteins; glycosyltransferase; human tissue; laminin; monoclonal antibody; neoplasm /cancer immunodiagnosis; neoplastic cell; oligosaccharides; thrombospondins; tissue /cell culture; tumor antigens

Altered glycosylation of glycolipids and glycoproteins is frequently observed in tumor tissues and cultured cancer cell lines. Changes in expression of glycosyltransferases produce unique carbohydrate structures which can be used to differentiate tumor from normal tissue and in some cases alter the biological activity of adhesive proteins and receptors which bear the altered oligosaccharides. We are examining the specificity of some monoclonal antibodies that recognize oligosaccharide determinants on functionally important molecules and the structures of sulfated glycoconjugates produced by cancer cells which bind to adhesive proteins such as laminin and thrombospondin. Currently we are examining globo- series sulfated glycolipids produced in human breast cancer and carbohydrates recognized by monoclonal antibodies to human secretory component and sialyl Lewis a which recognize glycoproteins and glycolipids produced in colon adenocarcinomas.

糖脂和糖蛋白的改变的糖基化通常是 在肿瘤组织和培养的癌细胞系中观察到。 变化 糖基转移酶的表达产生独特的糖结构 其可用于区分肿瘤与正常组织， 病例改变了粘附蛋白和受体的生物活性 其携带改变的寡糖。 我们正在研究 一些识别寡糖决定簇的单克隆抗体 功能上重要的分子和结构的硫酸化 由癌细胞产生的与粘附蛋白结合的糖缀合物 如层粘连蛋白和血小板反应蛋白。目前，我们正在研究全球- 人乳腺癌中产生的一系列硫酸化糖脂， 抗人分泌型白细胞介素2单克隆抗体识别的糖 识别糖蛋白和糖脂的唾液酸刘易斯a 产生于结肠腺癌。