STUDIES ON THE STRUCTURE AND FUNCTION OF HEAT-LABILE TOXIN FROM BORDETELLA

博德氏菌不耐热毒素的结构和功能研究

• 批准号: 3811011
• 负责人: D L BURNS
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3811011
• 关键词: Bordetella pertussis; bacterial toxins; endotoxins; free fatty acids; guinea pigs; heat; host organism interaction; immunologic skin test; ischemia; pertussis; protein structure function; species difference

Heat-labile toxin (HLT) is a potent toxin produced by Bordetella species. This toxin induces tissue damage and may contribute to damage observed after infection. Only one other toxin, tracheal cytotoxin, is known to be produced by all four species in the genus Bordetella. Since the different species produce similar diseases albeit in different hosts, HLT may play an important role in the pathogenesis of the organisms. We have initiated a program to examine the structure and function of this toxin. We have found that purified HLT induced ischemia in guinea pig skin. When endotoxin contaminates the HLT preparation, the lesions produced are dermonecrotic rather than ischemic in nature. Thus the dermonecrotic lesions that have long been thought to be the hallmark of HLT action are only produced by rather crude preparations of the toxin which contain endotoxin. We have also found that free fatty acids inhibit HLT action. Work is continuing to determine the mechanism of this inhibition.

不耐热毒素(HLT)是由波氏杆菌产生的一种强效毒素 物种。这种毒素会导致组织损伤，并可能导致损伤。 感染后观察。只有一种毒素，气管细胞毒素，是 已知由波尔德氏菌属的所有四个物种产生。自.以来 不同的物种会产生相似的疾病，尽管不同的物种 HLT可能在HLT的发病机制中起重要作用。 有机体。我们已经启动了一个项目来检查结构和 这种毒素的功能。 我们发现纯化的HLT可引起豚鼠皮肤缺血。 当内毒素污染HLT制剂时，损伤产生 本质上是皮肤坏死而不是缺血。因此， 长期以来，皮肤坏死性病变一直被认为是 HLT的作用只有在相当粗糙的毒素制剂中才能产生 含有内毒素。我们还发现，游离脂肪酸 抑制HLT的作用。工作仍在继续，以确定 这种抑制。