EVALUATIONS OF GENETIC TOXICITY TEST RESULTS

遗传毒性试验结果评价

• 批准号: 3918643
• 负责人: E ZEIGER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3918643
• 关键词: Rodentias; Salmonella; azo compounds; chemical carcinogen; chemical carcinogenesis; chlorohydrocarbon; chromosome aberrations; diagnosis design /evaluation; mutagen testing; neoplasm /cancer diagnosis; phthalates; toxicant screening

A database of results from a number of short-term assays has been created using chemicals tested by the CGTB. A subsection of this database contains chemicals tested for carcinogenicity by the NCI and the NTP. This database allows the evaluation of each short- term assay with respect to its ability to predict carcinogenesis or other short-term assay results. It also permits studies of the individual assays with respect to inter- and intra-laboratory reproducibility, and the effects of protocol changes on test responses. As a followup on an evaluation of the ability of four short-term tests (Salmonella mutagenicity; L5178Y mouse lymphoma call mutagenicity; in vitro chromosome aberrations in CHO cells; and in vitro sister chromatid exchanges in CHO cells) to predict carcinogenicity using 73 chemicals, an additional 40 chemicals are being examined. These results will be used to determine if the results obtained with the original 73 chemicals were representative of the database as a whole. In addition to examining the qualitative predictivity of the assays, a study has been initiated to examine the predictivity of the assays as a function of the potency of the short-term test and carcinogenicity test responses. More than 300 coded chemicals have been tested for mutagenicity in Salmonella in more than ore laboratory, or at different times in the same laboratory. The inter- and intra-laboratory reproducibility of the assay is being examined, and differences in qualitative responses will be evaluated with respect to possible differences in test protocols. These analyses will lead to an assessment of the ability of short-term genetic toxicity assays to predict rodent carcinogenicity, and to the design of test protocols and strategies for the deployment of short-term tests.

一个数据库的结果，从一些短期测定已被 使用CGTB测试过的化学物质制造。 其中的一个小节 数据库包含NCI测试的致癌性化学品 和NTP。 该数据库允许评估每个短- 关于其预测致癌作用能力的术语分析 或其它短期测定结果。 它还允许研究 实验室间和实验室内的单个检测 重现性，以及方案变更对试验的影响 应答 作为对四个短期能力评价的后续行动， 试验（沙门氏菌致突变性; L5178Y小鼠淋巴瘤检测 致突变性; CHO细胞中的体外染色体畸变;以及 CHO细胞中的体外姐妹染色单体交换）来预测 致癌性使用73种化学品，另外40种化学品是 正在接受检查。 这些结果将用于确定 用最初的73种化学品获得的结果具有代表性 数据库作为一个整体 除了检查 由于测定的定性预测性，已启动一项研究 检查测定的预测性， 短期试验和致癌性试验反应的效力。 超过300种编码的化学品已被测试的诱变性， 沙门氏菌在多个矿石实验室，或在不同的时间， 同一个实验室。 实验室间和实验室内 正在检查测定的重现性， 定性反应将根据可能的 测试协议的差异。 这些分析将导致 评估短期遗传毒性试验的能力， 预测啮齿动物致癌性，并设计测试方案 以及短期测试部署的策略。