LIPID METABOLISM OF THE MACACA FASCICULARIS

食蟹猴的脂质代谢

• 批准号: 3937273
• 负责人: D BARNARD
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3937273
• 关键词: Macaca; dietary lipid; enzyme mechanism; erythrocyte membrane; erythrocytes; fatty acids; feces analysis; lipid metabolism; nutrient bioavailability; nutrition related tag; veterinary medicine

The study will examine the metabolism of exogenous fatty acids by Macaca fascicularis. Trans fatty acids which cannot be synthesized by eukaryotes will be used as the exogenous fatty acid marker. These fatty acids are geometrical isomers of cis fatty acids which can be synthesized by eukaryotes. The trans fatty acids are catabolized and removed from the body shortly after the fatty acids are removed from the diet. Twelve male M. fascicularis monkeys were used in the study. Six were fed the control diet, and the others received the experimental diet. The RBC membrane of monkeys fed the control diet contained .5% trans octadecanoate isomers of the fatty acids. Examination of the fecal fatty acids from these monkeys showed that biohydrogenation does occur in their gut producing trans fatty acids which appear to be incorporated into the RBC membrane. Within 6 weeks after initiating the study the RBC membranes of monkeys fed the experimental diet contained 10.8% trans octadecanoate isomers of the total fatty acids. Corresponding with the increase in trans fatty acids there was an increase in the membrane linoleate to arachidonate ratio. This indicated that trans fatty acids interfere with arachidonate synthesis. There was also a displacement of saturated fatty acids and cis octadecanoate isomers. The double bond index was calculated and indicated that the observed perturbations in the fatty acid content of the RBC membranes from monkeys fed the experimental diet resulted in decreased membrane fluidity.

该研究将通过以下方式检查外源脂肪酸的代谢： 食蟹猴。 无法合成的反式脂肪酸 真核生物将用作外源脂肪酸标记。 这些脂肪酸是顺式脂肪酸的几何异构体， 可由真核生物合成。 反式脂肪酸是 脂肪酸在不久后被分解代谢并从体内排出 从饮食中去除。 十二只雄性束状猴 研究中使用了。 六人喂食对照饮食，而 其他人则接受实验饮食。 猴子的红细胞膜 饲喂含有 0.5% 反式十八酸酯异构体的对照饮食 脂肪酸。 检查这些粪便中的脂肪酸 猴子表明生物氢化确实发生在它们的肠道中 产生反式脂肪酸，似乎并入 红细胞膜。 研究开始后 6 周内 喂食实验饲料的猴子的红细胞膜含有 10.8% 总脂肪酸的反式十八烷酸酯异构体。 与反式脂肪酸的增加相对应的是 膜亚油酸与花生四烯酸的比例增加。 这 表明反式脂肪酸干扰花生四烯酸 合成。 饱和脂肪酸也发生了置换 和顺式十八酸酯异构体。 双键指数为 计算并表明观察到的扰动 喂食猴子的红细胞膜的脂肪酸含量 实验饮食导致膜流动性降低。