LIPID METABOLISM OF THE MACACA FASCICULARIS

食蟹猴的脂质代谢

• 批准号: 3916159
• 负责人: D BARNARD
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916159
• 关键词: Macaca; cell membrane; dietary lipid; enzyme mechanism; erythrocyte membrane; erythrocytes; fatty acids; feces analysis; fluid; lipid metabolism; nutrient bioavailability; nutrition related tag; veterinary medicine

The study will examine the metabolism of exogenous fatty acids by Macaca fascicularis. Trans fatty acids which cannot be synthesized by eukaryotes will used as the exogenous fatty acid marker. These fatty acids are geometrical isomers of cis fatty acids which can be synthesized by eukaryotes. The trans fatty acids are catabolized and removed from the body shortly after the fatty acids are removed from the diet. Twelve ale M. fascicularis monkey were used in the study. Six were fed the control diet, and the others received the experimental diet. The RBC membrane of monkeys fed the control diet contained .5% trans octadecanoate isomers of the fatty acids. Examination of fecal fatty acids from these monkeys showed that biohydrogenation does occur in their gut producing trans fatty acids which appear to be incorporated into the RBC membrane. Within 6 weeks after initiating the study the RBC membranes of monkeys fed the experimental diet contained 10.8% trans octadecanoate isomers of the total fatty acids. DPII steady state fluorescence polarization analysis of the erythrocyte membrane fluidity showed no change in membrane fluidity between the monkeys fed the two diets. However, insulin binding activity was altered in the monkeys fed the diet containing trans fatty acids.

这项研究将研究猕猴对外源脂肪酸的代谢 束状肌。真核生物不能合成的反式脂肪酸 将其作为外源脂肪酸的标记。这些脂肪酸是 顺式脂肪酸几何异构体的合成 真核生物。反式脂肪酸被分解并从 在脂肪酸从饮食中移除后不久。十二杯啤酒 本研究以束支原体为研究对象。六个人被喂给对照组 饮食，其他人接受实验饮食。红细胞膜 喂食对照饲料的猴子含有0.5%的反式十八酸异构体 脂肪酸。这些猴子粪便中脂肪酸的检测 表明它们的肠道中确实发生了生物氢化反应，产生反式脂肪 似乎被结合到红细胞膜中的酸。在6小时内 在研究开始几周后，给猴子喂食红细胞膜 试验日粮中含有10.8%的反式十八酸异构体 脂肪酸。DPII稳态荧光偏振分析 红细胞膜流动性无明显变化。 猴子给这两种食物喂食。然而，胰岛素结合活性是 在喂食含有反式脂肪酸的饮食的猴子中发生了变化。