LIPID METABOLISM OF THE MACACA FASCICULARIS

本研究将探讨猕猴对外源性脂肪酸的代谢 束状的 真核生物不能合成的反式脂肪酸 将用作外源性脂肪酸标记物。 这些脂肪酸 顺式脂肪酸的几何异构体， 真核生物 反式脂肪酸被分解代谢，并从 在脂肪酸从饮食中去除后不久， 十二麦芽酒 M. fascicularis猴用于研究。 其中六只喂食对照组 饮食，其他人接受实验饮食。 红细胞膜 喂食对照饮食的猴子含有0.5%的反式十八烷酸酯异构体 脂肪酸。 这些猴的粪便脂肪酸检查 表明生物氢化确实发生在它们的肠道中， 这些酸似乎被掺入RBC膜中。 仅6 研究开始后几周， 实验饮食含有10.8%的反式十八烷酸异构体的总 脂肪酸 DPII稳态荧光偏振分析 红细胞膜流动性无明显变化， 猴子喂食两种饮食。 然而，胰岛素结合活性是 在喂食含有反式脂肪酸的食物的猴子中发生了改变。

The study will examine the metabolism of exogenous fatty acids by Macaca fascicularis. Trans fatty acids which cannot be synthesized by eukaryotes will used as the exogenous fatty acid marker. These fatty acids are geometrical isomers of cis fatty acids which can be synthesized by eukaryotes. The trans fatty acids are catabolized and removed from the body shortly after the fatty acids are removed from the diet. Twelve ale M. fascicularis monkey were used in the study. Six were fed the control diet, and the others received the experimental diet. The RBC membrane of monkeys fed the control diet contained .5% trans octadecanoate isomers of the fatty acids. Examination of fecal fatty acids from these monkeys showed that biohydrogenation does occur in their gut producing trans fatty acids which appear to be incorporated into the RBC membrane. Within 6 weeks after initiating the study the RBC membranes of monkeys fed the experimental diet contained 10.8% trans octadecanoate isomers of the total fatty acids. DPII steady state fluorescence polarization analysis of the erythrocyte membrane fluidity showed no change in membrane fluidity between the monkeys fed the two diets. However, insulin binding activity was altered in the monkeys fed the diet containing trans fatty acids.