LIPID METABOLISM OF THE MACACA FASCICULARIS

食蟹猴的脂质代谢

• 批准号: 3959913
• 负责人: D BARNARD
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3959913
• 关键词: dietary lipid; erythrocyte membrane; erythrocytes; fatty acids; feces analysis; lipid metabolism; nutrient bioavailability; nutrition related tag

The study will examine the metabolism of exogenous fatty acids by Macaca fascicularis. Trans fatty acids which cannot be synthesized by eukaryotes will be used as the exogenous fatty acid marker. These fatty acids are geometrical isomers of cis fatty acids which can be synthesized by eukaryotes. The trans fatty acids are catabolized and removed from the body shortly after the fatty acids are removed from the diet. Twelve male M. fascicularis monkeys were used in the study. Six were fed the control diet, and the others received the experimental diet. The RBC membrane of monkeys fed the control diet contained .5% trans octadecanoate isomers of the fatty acids. Examination of the fecal fatty acids from these monkeys showed that biohydrogenation does occur in their gut producing trans fatty acids which appear to be incorporated into the RBC membrane. Within 6 weeks after initiating the study the RBC membranes of monkeys fed the experimental diet contained 10.8% trans octadecanoate isomers of the total fatty acids. Corresponding with the increase in trans fatty acids there was an increase in the membrane linoleate to arachidonate ratio. This indicated that trans fatty acids interfere with arachidonate synthesis. There was also a displacement of saturated fatty acids and cis octadecanoate isomers. The double bond index was calculated and indicated that the observed perturbations in the fatty acid content of the RBC membranes from monkeys fed the experimental diet resulted in decreased membrane fluidity.

这项研究将检查外源性脂肪酸的代谢 被食蟹猴杀死 反式脂肪酸不能 由真核生物合成的脂肪酸将被用作外源脂肪酸 标记。 这些脂肪酸是顺式脂肪酸的几何异构体， 真核生物可以合成的酸。 反式脂肪 酸被分解代谢，并从身体后不久， 从饮食中除去脂肪酸。 12只雄性M. 在研究中使用食蟹猴。 六个人吃了 控制饮食，其他人接受实验饮食。 的 喂食对照饲料的猴子的RBC膜含有0.5%的 脂肪酸的反式十八烷酸酯异构体。 审查 这些猴子的粪便脂肪酸显示， 生物氢化确实发生在它们的肠道中， 这些酸似乎被掺入RBC膜中。 研究开始后6周内， 喂食实验饮食的猴子含有10.8%的反式 总脂肪酸的十八酸酯异构体。 对应 随着反式脂肪酸的增加， 膜亚油酸酯与花生四烯酸酯比率。 这表明 反式脂肪酸干扰花生四烯酸合成。 那里 也是饱和脂肪酸和顺式 十八烷酸酯异构体。 计算双键指数， 表明在脂肪酸中观察到的扰动 猴子的红细胞膜的含量 实验饮食导致膜流动性降低。