TRANSMEMBRANE WATER AND ION MEASUREMENT SYSTEM (TWIMS)

跨膜水和离子测量系统 (TWIMS)

• 批准号: 6411182
• 负责人: LID B WONG
• 金额: $ 9.65万
• 依托单位: BIOTECHPLEX CORPORATION
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2002-03-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6411182
• 关键词: biological fluid transport; biomedical equipment development; fluorescence; ion transport; measurement; optics; photomultiplier; water channel

DESCRIPTION (adapted from applicant's abstract): LiDon Technologies proposes to incorporate a nanosecond phase-frequency modulation fluorescence lifetime measurement technique to a bi-directional water flux measurement system developed by the principals of LiDon; the Trans-membrane Water and Ion Measurement System (TWIMS) (Philis, Wong & Yeates, 1999). The system is based on the measurement of the modulation of the temporal changes in the fluorescence of 8-amino-1,3,6-naphthalene tri-sulfonate (ANTS) in D2O, compared to H2O due to bi-directional transport of D2O and H2O across a membrane partition. ANTS is excited with 397 nm light emitted from a sinusoidally-modulated blue LED at MHz frequencies. The phase as well as the modulation factor of the emitted fluorescence at 514 nm are determined using a dual photomultiplier tube in conjunction with a cross correlation technique. In this Phase I feasibility project, LiDon will incorporate these electro-optical technologies to improve the sensitivity and temporal resolution of the system. In Phase II, the system will be further optimized and automated to enhance the novelty of the TWIMS while reducing its costs of production so that profitable systems can be manufactured to attain market competitiveness. The maintenance of homeostatic water transport across biological membranes is fundamental to life and abnormalities in trans-membrane transport lead to diseases in organs and tissues, including the lungs, intestine, kidneys, eyes, and brain. This system is targeted to a yet untapped market. This market is forecast to undergo substantial growth PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述（改编自申请人的摘要）：LiDon Technologies提出， 结合纳秒相位-频率调制荧光寿命 双向水流量测量系统的测量技术 由LiDon的负责人开发;跨膜水和离子 测量系统（TWIMS）（Philis、Wong和Yeates，1999年）。该系统是基于在 在测量调制的时间变化， 8-氨基-1，3，6-萘三磺酸盐（ANTS）在D2 O中的荧光，比较 由于D2 O和H2O跨膜的双向运输， 分区ANTS用从发射的397 nm光激发， 正弦调制的蓝色LED在MHz频率。阶段以及 在514 nm处发射的荧光的调制因子使用 双光电倍增管结合互相关技术。在 在第一阶段的可行性项目中，LiDon将把这些电光 技术，以提高系统的灵敏度和时间分辨率。 在第二阶段，该系统将进一步优化和自动化，以提高 TWIMS的新奇，同时降低其生产成本， 可以制造系统以获得市场竞争力。维护 水在生物膜上的稳态运输是 生命和跨膜转运的异常导致器官疾病 和组织，包括肺、肠、肾、眼和脑。这 该系统的目标是一个尚未开发的市场。预计该市场将经历 大幅增长 拟议商业应用：不可用