TRANSMEMBRANE WATER AND ION MEASUREMENT SYSTEM (TWIMS)

跨膜水和离子测量系统 (TWIMS)

• 批准号: 6411182
• 负责人: LID B WONG
• 金额: $ 9.65万
• 依托单位: BIOTECHPLEX CORPORATION
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2002-03-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6411182
• 关键词: biological fluid transport; biomedical equipment development; fluorescence; ion transport; measurement; optics; photomultiplier; water channel

DESCRIPTION (adapted from applicant's abstract): LiDon Technologies proposes to incorporate a nanosecond phase-frequency modulation fluorescence lifetime measurement technique to a bi-directional water flux measurement system developed by the principals of LiDon; the Trans-membrane Water and Ion Measurement System (TWIMS) (Philis, Wong & Yeates, 1999). The system is based on the measurement of the modulation of the temporal changes in the fluorescence of 8-amino-1,3,6-naphthalene tri-sulfonate (ANTS) in D2O, compared to H2O due to bi-directional transport of D2O and H2O across a membrane partition. ANTS is excited with 397 nm light emitted from a sinusoidally-modulated blue LED at MHz frequencies. The phase as well as the modulation factor of the emitted fluorescence at 514 nm are determined using a dual photomultiplier tube in conjunction with a cross correlation technique. In this Phase I feasibility project, LiDon will incorporate these electro-optical technologies to improve the sensitivity and temporal resolution of the system. In Phase II, the system will be further optimized and automated to enhance the novelty of the TWIMS while reducing its costs of production so that profitable systems can be manufactured to attain market competitiveness. The maintenance of homeostatic water transport across biological membranes is fundamental to life and abnormalities in trans-membrane transport lead to diseases in organs and tissues, including the lungs, intestine, kidneys, eyes, and brain. This system is targeted to a yet untapped market. This market is forecast to undergo substantial growth PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述（根据申请人的摘要改编）：Lidon Technologies建议 结合纳秒相频率调制荧光寿命 双向水通量测量系统的测量技术 由Lidon校长开发；跨膜水和离子 测量系统（Twims）（Philis，Wong＆Yeates，1999）。该系统基于 关于测量时间变化的调制 D2O中的8-氨基-1,3,6-萘三磺酸盐（ANT）的荧光比较 由于D2O和H2O跨膜的双向运输而到H2O 分割。蚂蚁用从A发出的397 nm光激发 正弦调节的蓝色LED在MHz频率下。阶段以及 使用A确定在514 nm处发射荧光的调节因子 双光电倍增管与跨相关技术结合使用。在 这阶段的I阶段可行性项目，Lidon将结合这些电源 提高系统灵敏度和时间分辨率的技术。 在第二阶段，系统将进一步优化并自动化以增强 Twims的新颖性，同时降低其生产成本，从而获利 可以制造系统以获得市场竞争力。维护 跨生物膜的稳态水运输是至关重要的 跨膜运输的生命和异常导致器官疾病 和组织，包括肺，肠，肾脏，眼睛和大脑。这 系统针对尚未开发的市场。这个市场预计将经历 实质性增长 拟议的商业应用程序：不可用