TRANS-MEMBRANE WATER AND ION MEASUREMENT SYSTEM (TWIMS)

跨膜水和离子测量系统 (TWIMS)

• 批准号: 6765310
• 负责人: LID B WONG
• 金额: $ 37.5万
• 依托单位: BIOTECHPLEX CORPORATION
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6765310
• 关键词: biological fluid transport; biomedical equipment development; electrophysiology; fluorescence; ion transport; measurement; optics; photomultiplier; sheep; water channel; water flow

DESCRIPTION (provided by applicant): BioTechPlex proposes to develop a Transmembrane Water and Ion Measurement System (TWIMS) to measure miniscule water fluxes across biological membranes in conjunction with their electro-physiological properties. BioTechPlex has accomplished all the objectives proposed in Phase I. A) Developed a novel light source and associated optics for fluorescence photon detection and analysis. B) Integrated the bench optics into a measurement system, tested the system and demonstrated its markedly increased sensitivity for the measurement of transmembrane water fluxes. In this Phase II project, BioTechPlex will integrate the principle and concepts of the electro-optics developed in this Phase I to build an 8-tissue chamber system. To develop a manufacturability prototype of the TWIMS, we will proceed with the development of further innovative technologies. These include: 1) fluorescence detection technology; 2) prototyping of the chambers and tissue holders to accommodate both native epithelia and confluent tissue cultures; and 3) a virtual instrumentation platform for the measurement of luminal to basolateral and basolateral to luminal waters fluxes, potential difference and short circuit current. This multichamber system will be designed to suit the needs of physiologists and pharmacologists, in academia, government and industry. It will be designed for scientific research experiments as well as for use in the drug discovery industry. BioTechPlex plans to market the TWIMS for scientific investigations and drug discovery in fields including but not limited to, 1) respiratory disease such as chronic bronchitis, asthma bronchiectasis and cystic fibrosis; 2) gastrointestinal disease such as diarrhea, cholera infection and severe dehydration; 3) kidney disease such as renal failure; and 4) eye diseases such as dry eye syndrome.

描述（由申请人提供）： Biotechplex建议开发跨膜水和离子测量系统（TWIMS），以测量跨生物膜的微型水通量以及它们的电生理特性。 Biotechplex完成了第I阶段提出的所有目标。A）开发了一种新型的光源和相关的光学器件，用于荧光光子检测和分析。 b）将基准光学元件整合到测量系统中，测试了该系统，并证明了其对跨膜水通量测量的敏感性显着提高。在此II阶段项目中，Biotechplex将整合本I阶段在I阶段开发的电气原理的原理和概念，以构建8组织室内系统。为了开发Twims的制造性原型，我们将继续开发进一步的创新技术。其中包括：1）荧光检测技术； 2）室和组织持有人的原型制作，以适应天然上皮和汇合组织培养物； 3）一个虚拟仪表平台，用于测量腔内到基底外侧和基底外侧到腔水的通量，电势差和短路电流。该多学院系统将旨在满足学术界，政府和工业的生理学家和药理学家的需求。它将用于科学研究实验以及在药物发现行业中的使用。 Biotechplex计划在包括但不限于，1）呼吸道疾病（例如慢性支气管炎，哮喘支气管扩张和囊性纤维化）中销售Twims进行科学研究和药物发现； 2）胃肠道疾病，例如腹泻，霍乱感染和严重脱水； 3）肾脏疾病，例如肾衰竭； 4）眼部疾病，例如干眼综合征。