FIBER OPTIC LASER DOPPLER SYSTEM FOR CILIARY MOTILITY

用于肺细胞监测的无创性传感器和探头的发展 体内功能将为病理生理学提供新的信息 肺部疾病。这将使采取预防性措施或 治疗干预方法将基于以下方面开发 测量到的细胞异常。在这个第一阶段的提案中，活体内， 无中断、光纤激光多普勒系统(FLODS)将 用于实时测量睫毛活动的两个参数 即纤毛搏动频率(CBF)和异时波周期(MWP)。 这些参数是有效的粘液纤毛运输的基础。这个 传感探头将通过光纤的采样通道 支气管镜检查。将开发一种光纤原型，利用 动态激光光散射与时频的关系 和时间尺度分析(小波)。CBF和MWP将在#年确定 天然纤毛上皮和狗的纤毛上皮。在第二阶段，该系统将 被打包并在人体上进行测试。我们设想这将被用于 常规气管支气管镜和鼻内窥镜下上颌骨副鼻腔镜术 在门诊护理病房和医院对病人进行检查。它 将提供首个活体内的定量信息。这将是 支持定性可视化临床评估，消除了 用于体外测定脑血流量的活组织检查。 建议的商业应用 该系统将用于鼻部和肺部疾病的展示 粘液纤毛运输障碍。该系统也适用于 评估大多数类型的生物运动，流速分布 流体以及物理振动的远程测量。

Development of non-invasive sensory and probes to monitor lung cell function in vivo will provide novel information on the pathophysiology of lung diseases. This will enable rationales for prophylactic or therapeutic intervention approaches to be developed based on the measured cellular abnormality. In this Phase I proposal, an in vivo, non-disruptive, Fiber Optics Laser Doppler system (FOLDS) will be constructed to measure two parameters of ciliary activity in realtime namely, ciliary beat frequency (CBF) and metachronal wave period (MWP). These parameters are fundamental to effective mucociliary transport. The sensing probe will pass through the sampling channel of a fiber-optic broncho-endoscope. A fiberoptic prototype will be developed utilizing dynamic laser light scattering in conjunction with the time-frequency and time-scale analyses (wavelet). CBF and MWP will be determined in native ciliated epithelia and in dogs. In Phase II, this system will be packaged and tested in humans. We envision that this will be used during routine trachael-bronchoscopy and endoscopic paranasal maxillary examination in patients in ambulatory care units and in hospitals. It will provide the first in vivo quantitative information. This will support qualitative visual clinical assessment and eliminate the need for biopsies for in vitro determination of CBF. PROPOSED COMMERCIAL APPLICATION This system will be used in nasal and lung diseases that exhibit impairment of mucociliary transport. The system is also applicable to assess most types of biological motilities, velocity flow profile of fluids as well as remote measurements of physical vibrations.