High Content Screening for Epithelial Biology

上皮生物学高内涵筛选

• 批准号: 6792940
• 负责人: LID B WONG
• 金额: $ 5.61万
• 依托单位: BIOTECHPLEX CORPORATION
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2005-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6792940
• 关键词: Content; Screening; Epithelial; Biology

DESCRIPTION (provided by applicant): BioTechPlex Corporation (BioTechPlex TM) proposes to develop a high content cellular screening system for epithelial cellular physiology. This system will enable the kinetic responses of multiple cellular fluorophore reportable molecular species to be simultaneously measured in a 96 well plate. The kinetic responses will be measured in a millisecond time frame, consistent with the temporal nature of many intracellular kinetic processes. This technology will provide a novel, multi-probe cellular-based platform for high content screening and high-throughput drug screening. This enabling technology will provide a major tool for the elucidation of cell based signal transduction mechanisms for drug discovery and validation by the pharmaceutical industry.

描述（由申请人提供）：Biotechplex Corporation（Biotechplex TM）提议开发用于上皮细胞生理学的高含量细胞筛选系统。该系统将使多个细胞荧光团可报告的分子物质的动力学反应同时在96井板中测量。动力学响应将以毫秒的时间框架进行测量，与许多细胞内动过程的时间性质一致。这项技术将为高素质筛查和高通量药物筛查提供一种新型的，多探针的基于蜂窝的平台。这种启示技术将为制药行业阐明用于药物发现和验证的基于细胞的信号转导机制的主要工具。