FIBER OPTIC LASER DOPPLER SYSTEM FOR CILIARY MOTILITY

LiDon Technologies （LiDon）提出开发一种系统，用于测量人体气管和支气管以及鼻窦中控制纤毛粘膜有效运输的纤毛活动的两个基本参数，即纤毛搏动频率（CBF）和超向波周期（MWP）。光纤激光多普勒系统传感探头将通过光纤支气管镜的采样通道插入。通过新技术的结合，LiDon将生产出第一个在体内进行这些测量的商业可用系统。LiDon将承担必要的研发工作，推出3个版本的fold系统。fold -1：一种体外系统，用于实时收集培养细胞和天然上皮细胞中的CBF和MWP数据。fold -2：一种体内系统，用于实时测量大小动物气管和支气管直径为3mm的CBF和MWP。FOLDS -3：一种体内系统，用于实时测量上呼吸道和下呼吸道的CBF和MWP。作为一项使能技术，fold将有助于对细胞、组织、动物和人类的纤毛活性进行定量评估，用于毒理学研究、药理学研究、肺部药物发现以及临床诊断和治疗。拟议的商业应用：该系统将用于鼻和肺部疾病，表现出粘膜纤毛运输障碍。该系统还适用于评估大多数类型的生物运动、流体的速度流动剖面以及物理振动的远程测量。

LiDon Technologies (LiDon) proposes to develop a system to measure, in the human tracheal and bronchial airways as well as in the paranasal sinuses, two fundamental parameters of ciliary activity governing effective mucociliary transport, namely, ciliary beat frequency (CBF) and metachronal wave period (MWP). The Fiber Optics Laser Doppler System (FOLDS) sensing probe will be inserted through the sampling channel of a fiber optic bronchoscope. Through the incorporation of novel technologies LiDon will produce the first commercially available system to make these measurements in vivo. LiDon will undertake the R & D necessary to launch 3 versions of the FOLDS system. FOLDS -1: An in vitro system designed to make realtime data collection of CBF and MWP in cultures and in native epithelia. FOLDS -2: An in vivo system designed to make realtime measurements of CBF and MWP in the trachea and bronchial airways to 3 mm diameter in small and large animals. FOLDS -3: An in vivo system designed to make realtime measurements of CBF and MWP in the upper and lower human respiratory tract. The FOLDS, being an enabling technology, will facilitate the quantitative assessment of ciliary activity in cells, tissues, animals and humans for toxicological studies, pharmacology research, pulmonary drug discovery, and clinical diagnosis and therapeutic treatment. PROPOSED COMMERCIAL APPLICATION: This system will be used in nasal and lung diseases that exhibit impairment of mucociliary transport. The system is also applicable to assess most types of biological motilities, velocity flow profile of fluids and well as remote measurements of physical vibrations.