TRANS-MEMBRANE WATER AND ION MEASUREMENT SYSTEM (TWIMS)

跨膜水和离子测量系统 (TWIMS)

• 批准号: 6646144
• 负责人: LID B WONG
• 金额: $ 37.5万
• 依托单位: BIOTECHPLEX CORPORATION
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6646144
• 关键词: biological fluid transport; biomedical equipment development; electrophysiology; fluorescence; ion transport; measurement; optics; photomultiplier; sheep; water channel; water flow

DESCRIPTION (provided by applicant): BioTechPlex proposes to develop a Transmembrane Water and Ion Measurement System (TWIMS) to measure miniscule water fluxes across biological membranes in conjunction with their electro-physiological properties. BioTechPlex has accomplished all the objectives proposed in Phase I. A) Developed a novel light source and associated optics for fluorescence photon detection and analysis. B) Integrated the bench optics into a measurement system, tested the system and demonstrated its markedly increased sensitivity for the measurement of transmembrane water fluxes. In this Phase II project, BioTechPlex will integrate the principle and concepts of the electro-optics developed in this Phase I to build an 8-tissue chamber system. To develop a manufacturability prototype of the TWIMS, we will proceed with the development of further innovative technologies. These include: 1) fluorescence detection technology; 2) prototyping of the chambers and tissue holders to accommodate both native epithelia and confluent tissue cultures; and 3) a virtual instrumentation platform for the measurement of luminal to basolateral and basolateral to luminal waters fluxes, potential difference and short circuit current. This multichamber system will be designed to suit the needs of physiologists and pharmacologists, in academia, government and industry. It will be designed for scientific research experiments as well as for use in the drug discovery industry. BioTechPlex plans to market the TWIMS for scientific investigations and drug discovery in fields including but not limited to, 1) respiratory disease such as chronic bronchitis, asthma bronchiectasis and cystic fibrosis; 2) gastrointestinal disease such as diarrhea, cholera infection and severe dehydration; 3) kidney disease such as renal failure; and 4) eye diseases such as dry eye syndrome.

描述（由申请人提供）： 生物技术公司建议开发一种跨膜水和离子测量系统（TWIMS），以测量跨生物膜的微小水通量及其电生理特性。生物科技公司已经完成了第一阶段提出的所有目标。A）开发了用于荧光光子检测和分析的新型光源和相关光学器件。B）将工作台光学器件集成到测量系统中，测试该系统并证明其对于跨膜水通量的测量的显著增加的灵敏度。在第二阶段的项目中，生物技术公司将整合第一阶段开发的电光原理和概念，建立一个8组织室系统。为了开发TWIMS的可制造原型，我们将继续开发进一步的创新技术。其中包括：1）荧光检测技术; 2）腔室和组织保持器的原型设计，以容纳天然上皮细胞和汇合的组织培养物;以及3）虚拟仪器平台，用于测量管腔到基底外侧和基底外侧到管腔沃茨通量、电势差和短路电流。该多室系统的设计将满足学术界、政府和工业界生理学家和药理学家的需求。它将被设计用于科学研究实验以及用于药物发现行业。生物科技公司计划将TWIMS用于科学研究和药物发现领域，包括但不限于：1）呼吸系统疾病，如慢性支气管炎、哮喘、支气管扩张和囊性纤维化; 2）胃肠道疾病，如腹泻、霍乱感染和严重脱水; 3）肾脏疾病，如肾衰竭; 4）眼科疾病，如干眼综合征。